ANTI-CYTOKERATIN 20

Mab to Cytokeratin 20

cat#RDI-PRO61033 $410.00/vial

Clone Determination IT-Ks 20.5

Category Mouse monoclonal

Immunoglobulin Class IgG2a

Purification Protein A affinity chromatograph

Antigen Electrophoretically purified cytokeratin (CK) 20 from human intestinal mucosa

Clinical Value IT-Ks 20.5 represents an excellent marker for certain types of carcinomas such as adeno-carcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric fovelar epithelium, urothelial umbrella cells, Merkel cells of epidermis as metastatic colorectal carcinoma. Adenocarcinomas of breast, lung, endothelium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (cytokeratin#20, Mr 46,000)

Application Suitable for frozen tissue sections and cytological material

Immunoblotting (Western)

Stability/Storage at 2-8°C one year after reconstitution when stored at 2-8 DEG C. For extended storage, keep in aliquots at -20 DEG C. Avoid repeated thawing and freezing.

Quantity 50 µg (lyoph.) from PBS which contains 0.5%BSA and 0.09% NaN3

Reconstitution: Add 1ml DI Water

Polypeptide Reacting Protein IT (cytokeratin No. 20; Mr 46 000)

Antigen Recognized in Human (NOT with rat)

Reactivities on Cultured cell lines tested so far:

HT-29, LoVo, DLD-1, SW 1116, Cell Lines ,CaCo-2, RT-4

Working Dilution Reconstitute in 1 ml dist. water and dilute 1:10 in PBS (pH 7.4) for immunohistochemical application.

Incubation Time 1 h at RT or over night at 4°C.

Literature on Cytokeratin 20