Rabbit anti-Rat Glycine Transporter (GLYT1) Polyclonal Antibody (IgG fraction)

Rat/Human/Mouse/bovine

cat# RDI-RTGLYT1abrX $469.00/vial

Presentation: 100ug affinity pure antibody lyophilized from a 0.1ml (1mg/ml) PBS stock with 0.1% BSA and 0.02% merthiolate).

Reconsitution: add 100ul sterile DI water , tighten cap, lightly vortex. allow to set at least 30 minutes at room temp. If desired, centrifuge for 1 minute at 1000rpm to concentrate antibody in vial.

Purification: peptide affinity purified.

Antigen: A 20 amino acid peptide sequence mapped within the cytoplasmic, C terminal domain of rat GLYT1. The petide was conjugated to KLH

Reconstitution: All peptide/antibody received in solution or reconstituted from Lyophilized vials should be stored frozen at -20oC or below in suitable aliquots. It is not recommended to store diluted solutions. Avoid repeated freeze and thaw.

Specificity: The antigen sequence has 100% sequence homology to human GLYT1A, GLYT1b and GLY1c and 95% homology with mouse and bovine GLUT1. The peptide shows no significant homology with other known peptides.

USE: Western Blotting (1-10 ug/ml for affinity pure using Chemiluminescence technique).

ELISA (1:10K; using 50-100 ng of control peptide/well).

Histochemistry & Immunofluorescence:We recommend the testing of affinity purified antibody at 2-20ug/ml on acetone fixed frozen sections or pfa fixed & permeabilized tissues.

General References: -Mol. Pharmacol 45,608 (1994)

-Neuron 8, 927 (1992)

control/blocking peptide: cat#RDI-RTGLYT1-CP $188.00/100ug in 0.1ml (1mg/ml PBS pH 7.4 + 0.02% merthiolate)

Background: The glycine transporter belongs to a family of NA/Cl dependent neurotransmitter transporters, predicted to conatin as many as 12 transmembrane domains. Glycine receptor exists in 2 forms-GLYT1 and GLYT2. Form 1 has three other known isoforms (GLYT1a, GLYT1b, and GLYT1c) which may be produced by alternative splicing or promoter usage. Rodent GLYT1A and Glyt1b differ only by 10 amino acids at the N-terminus and expressed from the same gene. Although the N terminal part of mouse and rat GLYt1a is identical in mouse and rat, the N-terminus of Glyt1b are significantly different in these two species. The Glyt1c subtype has only been reported in humans. Glyt1a is expressed in CNS and peripheral organs. Glyt1b is localized in the CNS. Both neuron and glial cells have Glyt1. More recent studies indicate that Glyt1b may not be brain specific. Immunolocalization studies on Glyt2 suggest that Glyt2 is responsible for terminating the neurotransmission at the strychnine synapses.

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS

see also rabbit anti-rat GLYt2 cat#RDI-RTGLYT2abrx $469.00/100ug

20aa peptide derived, c terminus (cytoplasmic) rat reactive, no cross with glyt1.

see also related antibodies to Vesicular Monoamine Transporters, Acetylcholine, Creatine, Norepinephrine, Proline, Serotonin and Taurine Transporter