A proprietary polymer of HRP (horseradish peroxidase) conjugated to Streptavidin in various polymerization ranges. (20, 40 & 80). The POLY-HRP-SA product line can be used with virtually any biotin labeled product including probes & primary and secondary antibodies. It can replace the conventional streptavidin-HRP conjugates currently in use. It will activate your current HRP substrates including the sensitive TMB products (ELISA and blotting), DAB, luminol etc.... ?
What is POLY-HRP80-GAM:
A new specialty conjugate made with our POLY-HRP80 base. It is a goat anti-mouse (IgG + IgM) affinity absorbed antibody that is specially conjugated with our POLY-HRP 80 molecule. Its applications include sensitive histochemical applications and for use in various immunoassays. ? How does it work: With conventional conjugates, you have only one or two HRP molecules available to react with your substrate. With POLY-HRP conjugates you increase the number of HRP molecules by 5X, 10X and even more (depending upon the polymerization range used). The more HRP molecules available, the greater the activation of your substrate=the more sensitivity you obtain! ?
How is it supplied: The POLY-HRP-SA is supplied in a concentrated form of approx 1 milligram conjugate in 1ml of 50% glycerol/ buffer. It should be stored at -20 DEG C and fresh dilutions made daily. The undiluted stock is good for at least one year. The working dilution required will depend upon your assay characteristics. We recommend starting dilutions of 1:500 for blotting and 1:2000 for ELISA work. Working titers can then be derived to optimize your assay. -POLY-HRP80-GAM is supplied as approx 200ug conjugate in 1ml of 50% glycerol/buffer. It should also be stored at -20 DEG C with fresh dilutions made daily. It must be titered for each application with initial 1:10-1:100 dilutions for histochemistry