MOUSE MONOCLONAL ANTIBODY TO: HUMAN CALGRANULIN A

PRODUCT CODE RDI-CBL269 $375.00

CLONE CF-145

ISOTYPE IgG2b

SOURCE Mouse ascitic fluid

PURIFICATION METHOD

Ion-exchange chromatography

SPECIFICITY The antibody recognizes calgranulin A (S100 calcium-bindingProtein A8) which is expressed by macrophages in chronic inflammation. It is also found in granulocytes and in subset ofnormal stratified squamous epithelia including tongue,oesophagus and buccal cells. Calgranulin is found in elevatedlevels in the serum of cystic fibrosis cases and is also expressed in the skin of patients with psoriasis,eczematous dermatitis, some GVDH and squamous cell carcinoma.

APPLICATIONS * The antibody is suitable for use on formalin fixed paraffin sections where it may be seen as a marker for reactive epthelium in skin and for skin squamous cell carcinomas.* Flow cytometry.* ELISA assay.

REFERENCES Dorin J.R. et al. (1990) Genomics 8: 420-426.Dorin J.R. et al .(1987 Nature 326 (6133): 614-617Van Heyningen V. et al. (1985), Nature 315 (6019) 513-515Wilkinson M.M. et al. (1988) J. Cell Science 91:221-230

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.