Mouse anti-Na+/K+ BETA subunit Monoclonal ab (M17-P5-F11)
cat# RDI-atpBM17abm $469.00
Background: The NA+/K+ Atpase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of NA and K across the cell membrane. The enzyme consists of a large multipass transmembrane catalytic subunit (alpha subunit) and an associated smaller glycoprotein (Beta subunit). Studies indicate that there are three isoforms of the alpha subunit (A1, a2, & A3) and two isoforms of the Beta subunit (B1 & B2) which are encoded by two multigene families. The different isoforms of the NA/K ATPase exhibit tissue specific and developmental pattern of expression. The Alpha 1 and Beta mRNAs are present in all cell types examined whereas the Alpha-2 and Alpha-3 mRNAs exhibit a more restricted pattern of cell-specific expression.
isotype: mIgG2a
clone: M17-P5-F11
package: 200ul of diluted ascites fluid with 0.05% NaN3
antigen: produced from a fusion of SP20/0-Ag14 myeloma cells and spleen cells from F1 hybrid mice immunized with purified lamb kidney Beta subunit Na+ K+ Atpase.
Specificity: Beta 1 subunit of NA+/K+ ATPase -in human, sheep, canine and pig.
-binds to region between amino acids 195 and 199 of the sheep BETA 1 NA+ K+ ATPase.
Use: -western blot (detects a band at approx 42kDa in liver and approx 55 kDa in kidney samples) 1:250
-Immunohistochemistry: 1:100
ref: -Y. Sun and W.J. Ball, American Journal of Physiology, 262:C1491-C1499, 1992.
Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. BEFORE OPENING, suggest centrifuging for 1 minute at 1000 RPM to concentrate material in vial.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Monoclonal anti-NA-K-ATPASE, Beta-1 subunit
Catalog#: RDI-NAKATPB1abm Price: $562.00/vial
Packaging: 200ug protein G purified mouse IgG2a 0.1M Tris-glycine pH 7.4 with 0.05% sodium azide). -After thawing, tighten cap and centrifuge for 1-2 minutes at 1000 RPM to concentrate antibody in vial. Further dilutions in Tris-glycine buffers or PBS with 0.05% NaN3 (containing 1% BSA or carrier protein recommended).
Species: mouse IgG2a
Clone: C464.8
Antigen: purified Na K ATPase isolated from rabbit kidney .
Specificity: specific for isoform of Na-K ATPase Beta 1 subunit in rabbit, RAT, human, bovine and canine.
Uses: Western blot: 0.05-1ug/ml dilution in blocking solution (TRis buffered saline containing 3% non fat dry milk . Detects differentially glycosylated isoforms of Na-K ATPase Beta 1 isoform at approx 55/60 kda in brain, heart and kidney microsomal preparations
-Do not boil microsomes (warm to 37 DEG C)
- IMMUNOHISTOCHEMISTRY: 10ug/ml
-Immunoprecipitation: 3ug
ref: -J. Biol Chem 268:14342, 1993
-Science 260:552, 1993
-Am J Physiol 267:F75, 1994
-Am J Physiol 266:C579, 1994
Storage: Store at -20 DEG C AVOID FREQUENT FREEZE THAW CYCLES. Centrifuge 1000 RPM for 1 minute before opening to concentrate material in bottom of vial