Mouse anti-human CD114 antibodies-1 clone

CATALOG# CLONE# Workshop Host Form Price

RDI-CD114-LM LMM741 VI mIgG1 purified $562.00

Catalog#: RDI-CD114-LM $562.00/vial

also available:PE labeled cat#RDI-CD114-LMPE $594.00/0.2mg

Package Size: 500ug purified in 1ml (0.5mg/ml) 10mM sodium phosphate pH7.2 with 150mM NaCl and 0.09% sodium azide. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits- avoid contact with acids.)

Prep: purified by Protein G from TCS

Species: mouse IgG1k

CLONE: LMM741 Workshop Code:VI, MA98

Activity: Reacts with the human granulocyte-colony stimulating factor (G-CSF) receptor. G-CSFR are expressed on the neutrophils, myeloid leukemias, endothelium, platelets and placenta. The immunogen for this antibody was the CH0-6A11 cell line which was generated by transfection of CHO cells with an expression vector containing a full length cDNA encoding the human G-CSF receptor. Binding approx position 332-346

Application: -Indirect immunofluorescence cell surface staining (flow cytometry) , Titer for each application -recommend approx 2ug/million cells, use Streptavidin:PE, positive staining on human peripheral blood granulocytes and TF-1 and U-937 cell lines but not with human peripheral blood lymphocytes and Raji and JY cell lines.

-western blot (on peripheral blood granulocytes=2 bands 130kDa and a minor band at 95kDa).

-immunoprecipitation

-blocking(azide free)

References:

1) Leucocyte Typing VI, Garland Press, NY 1997

2) Proc Natl Acad Sci USA 91:2985-2988 (1994)

Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.