MONOCLONAL ANTI-NEUROFILAMENT 160kD ANTIBODY

PRODUCT CODE :RDI-CBL214 $375.00/200ug vial

CLONE: BF10

ISOTYPE: IgG2a

SOURCE: Tissue culture supernatent.

PURIFICATION METHOD: Protein A affinity chromatography.

SPECIFICITY: The clone BF10 recognises the 160kD neurofilament polypeptide in a number of mammalian and avian species. Tumours recognized include phaeochromocytoma, paraganglioma, ganglioneuroblastoma and others of neuronal origin.

APPLICATIONS:

* Staining was strong on both frozen and paraffin embedded tissue sections.

* Strong staining of neuronal processes in variety of tissues of human, rat, chicken, pig and reptile origin.

REFERENCES:

Weber, K. et al. Cold Spring Harbour Symp. Quant. Biol. 47, 717 - 29 (1983).

Anderton, B.H. et al. Nature 298, 84 - 6 (1982).

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 200µg/2ml in phosphate buffered saline solution containing 10mM sodium azide and 1mg/ml BSA. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.