Mouse ANTI-HUMAN MILK FAT GLOBULES (HMFG2)

PRODUCT CODE :RDI-CBL201X $438.00/vial

CLONE: 3.14.A3

ISOTYPE: IgG1

SOURCE: Mouse ascitic fluid

SPECIFICITY: React with a mucin-like glycoprotein molecule expressed in milk-fat globule membrane. HMFG2 reacts with a different epitope on the milk fat globule mucin, one which is poorly expressed on normal lactating breast ,but strongly expressed on most primary and secondary breast cancers.

APPLICATIONS:

* The antibody reacts with not only breast, but other types of carcinoma. HMFG2 reacts with ductal carcinomas .

* Because the antibody reacts with other carcinomas it is useful in determining the origin of secondary tumours (e.g. to bone marrow) and in a panel of markers to epithelial cells antigens (such as epithelial keratin AE1/AE3) in different diagnosis of lymphoma (negative) and carcinoma (positive).

* Indirect immunoperoxidase or alkaline phosphatase staining of histological sections of frozen or formalin-fixed, paraffin embedded tissues.

* The antibodies have also been used in ELISA techniques for serum assay of the HMFG2 antigen in breast and ovarian tumours.

-approx working dilution 1:50 to 1:100, incubate 30 minutes room temp

PRESENTATION: Each monoclonal is delivered in a sealed vial . Reconstitute with 1ml distilled water. Contains 15mM sodium azide as a preservative. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.