Monoclonal anti-calbindin D-28K.
CAT#: RDI-CALBINDabm $438.00/vial
LOT#:
Form: Lyophilized ascites (no preservatives).
Quantity: 200 ul.
Reconstitution: with 200 ul bidistilled water.
Description: Monoclonal anti-calbindin D-28k is a mouse IgG produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut. The antibody was evaluated for specificity and potency:a) by indirect immunofluorescent or immunoperoxidase labelling as well as PAP and Biotin-Avidin labeling of cryostat or vibratome-sections of 4% paraformaldehyde fixed tissue; b) by immunoenzymatic labeling of immunoblots; c) by radioimmunoassay.
The product is a monoclonal antibody (McAB) against calbindin D-28k, and calcium-binding protein of the EF- hand family related to calmodulin and troponin-C. The antibody reacts specifically with calbindin D-28k in tissue originating from human, monkey, rabbit, rat, mouse and chicken (but probably not fish). This antibody does not cross-react with calretinin or other known calcium binding-proteins. This McAB specifically stains the 45Ca-binding spot of calbindin D-28k (MW 28'000. IEP 4.8) in a two-dimensional gel. In radioimmunoassay it detects calbindin D-28k with a sensitivity of 10 ng/assay and an affinity of 1.6 x 10(12) L/M.
Dilution: -Immunohistochemistry: 1:5000 - 1:10,000 with avidin- biotin methods.
-Immunoblots: 1:100 - 1:1,000.
We recommend that the optimal dilutions be determined by titration experiments.
Storage: For long storage, keep at -80'C (or at least -20'C). For continuous use keep at 4'C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
References: 1. Celio M.R. et al., Cell Calcium 11:599-602, 1990.
-For Research use Only
Rabbit anti-Calbindin D-28k [NEW BATCH]
CAT#: RDI-Calbindabr $470.00/vial
LOT#:
Form: Lyophilized antiserum (no preservatives).
Quantity: 200 ul.
Reconstitution: with 200 ul bidistilled water.
Description: This antiserum was produced against recombinant rat calbindin D-28k. It cross reacts with many other species, including humans. It can be used for immunohistochemistry and immunoblotting. In immunoblots it has 10% crossreaction with calretinin. Thus, depending on the dilution of the antiserum and/or sensitivity of the immunostaining, this other calcium- binding protein could be also revealed. In case of doubt, we suggest to use our monoclonal antibody cat#RDI-CALBINDabm directed against calbindin D-28k, which does not recognize calretinin.
dilutions: Immunohistochemistry: 1:10,000 - 1:20,000 with the avidin-biotin methods.
Immunoblots: 1:100 - 1: 1,000
We recommend that the optimal dilutions be determined by titration experiments.
Storage: Reconstitute by shaking very long and sucking with a Pasteur-pipette until the pellet is dissolved. Make small portions (e.g.2-5 ul). For long storage, keep at -80'C (or at least -20'C). For continuous use keep at 4'C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
Rabbit antibody to Calbindin D-9k.
CAT#: RDI-RTCALD9Kabr $470.00/vial
LOT#:
Form: Lyophilized antiserum
Quantity: 200 ul
Reconstitution: with 200 ul bidistilled water.
Description: This antibody was produced against rat recombinant Calbindin D-9k. It can be utilized in immunohistochemistry and in immunoblots.
Dilutions: Immunohistochemistry: 1:1,000 - 1: 10,000 with the avidin-biotin method.
Immunoblots: 1: 1,000.
We recommend that the optimal dilutions be determined by titration experiments.
Storage: For long storage, keep at -80'C (or at least -20'C). For continuous use keep at 4'C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
For Research Use Only
Calbindin-D9k (porcine intestine)
cat#RDI-CALBD9K-AG $500.00/200ug $438.00/vial 3+
White lyophilized solid. Intestinal vitamin D-dependent calcium-binding protein. Functions as a cytosolic buffer for Ca2+ ions in duodenal enterocytes and facilitates transepithelial active transport of Ca2+ ions. Also present in porcine uterus and placenta. Binds to two equivalents of Ca2+. Purity: >98% by HPLC. Sold on the basis of peptide content. Molecular Weight: 8840.0 Solubility: H2O Storage: Freezer (-20°C). This product is stable for 2 years as supplied.
Recombinant rat calbindin D-9K
cat#RDI-RTCALBD9k-AG $312.00/10ug $1000.00/60ug
Recombinant rat calbindin D-28K
cat#RDI-RTCALBD28K-AG $281.00/10ug $1000.00/100ug