rev: August 30, 2001

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Immunoasaay Enhancers

for Researchers and Manufacturers


NON AZIDE/NON Mercury Containing Preservatives


INTRODUCING: NON Azide/NON Mercury Containing ANTIMICROBIALS

FOR: Diagnostics   Molecular Biology    Immunoassay   Laboratory Reagents

Replacing: Thimerosal, Azide, Xylenols And Other Hazardous   Preservatives


RDI  is pleased to announce the availability of three new antimicrobials.
       (providied in forms and purity suitable for in vitro assays)

Safe for use and disposal * at recommended use levels, Microcide I, Microcide II and Microcide III do not contain mercury, azide or other dangerous materials. They do not contain or generate form-ldehyde. These preservatives provide broad-spectrum antimicrobial activity against bacteria and fungi and are compatible with most formulations.

*see safety data sheets with shipment-suitablility for use the responsibility of the end user


MICROCIDE I...........CAT# RDI-E448

   $250.00/ 100 grams

   $625.00/ 500 grams  

   $500.00/500 gram 2+

Microcide I has a boiling point of approximately 210'C, meaning that some material can be lost during lyophilization. This property has both advantages and disadvantages: it allow the Microcide I to be present while the reagent is in the liquid form; it then can be removed by lyophilization. If the antimicrobial is needed again later in the liquid reagent after reconstitution, Microcide I can be incorporated into the reconstituting solution.


MICROCIDE II..........CAT# RDI-E423

  $250.00/ 100 grams

  $625.00/ 500 grams

Microcide II is not removed during lyophilization, but since this antimicrobial has a positively-charged property, it can be complexed with very negatively-charged materials. It also has a small amount of surfactant (detergent) activity.


MICROCIDE III.........CAT# RDI-456

  $250.00/ 100 ml

   $500.00/ 500 ml

Microcide III is offered to complement the first two antimicrobials and is formulated for use in specialized applications. Chemically, Microcide III is not as stable as the other two anti-microbials. It is somewhat reactive against sulfhydryl and amino groups, and it also can be lost during lyophilization.


SAFETY (see safety data sheets sent with shipment, shipped as corrosive material)

Safe to use at recommended use levels

Safe for disposal at recommended use levels

Do not contain mercury, azide or other dangerous materials

Do not contain or generate formaldehyde

Replace thimerosal, sodium azide, chloramphenicol, etc.


ECONOMY

Low treatment costs

Low use levels


EFFICACY AND STABILITY

Microcide I and Microcide II show excellent stability over a

broad pH range

Broad-spectrum antimicrobial activities against bacteria and

fungi

Long-lasting activity

Development of resistance not observed

COMPATIBILITY

No alteration of product properties, color or performance in most systems

Compatible with most common formulations  Does not interfere with most enzyme assays, immunoassays, diag nostic reactions, ISE electrodes, restriction enzymes, electrophoresis applications. PCR-type reactions, nucleic acid purification, protein purification or buffer reagents



Characteristics Microcide I Microcide II Microcide III
catalog# RDI-E448 RDI-E423 RDI-E456
Purity >98% >99% solution
CAS# 3007-47-7 1119-97-7 Blend
common name 5 bromo-5 nitro 1,3 dioxane morpan T -------
Solubility (H2O) 0.5% 20.0% N/A
Stability (20 DEG C) >1 year >1 year > 1 year
Antimicrobial Activity:

  Fungi/Yeast

Gram + bacteria

Gram - bacteria

100ppm

100-300ppm

3-100ppm

10-30ppm

10-100ppm

300ppm

0.05%-0.09%

0.05-0.08%

0.05-0.08%

Recommended USE* 300ppm 300ppm 0.3%
Supplied as: white,

free flowing powder

white,

free flowing powder

clear

slighly amber solution

Melting Point 61.1 DEG C 232 DEG C N/A
Molecular weight 212.00 336.4 149.5
UV Max 224.5nm 218nm 273.0nm
pH (1.0%, H2O, 20 DEG C) 5.7 5.6 3.8
pH range (optimum) 5-9 4-9 2-9

*actual concentrations must be determined in your own system


TEST ORGANISMS:

Gram-positive Bacteria

Bacillus subtilis (ATCC 6633)

Streptococcus faecalis (clinical strain)


Gram-negative Bacteria

Escherchia coli (ATCC 11229*)

Enterobacter clocae (ATCC 23355)

Pseudomonas aeruginosa (ATCC 14207)


YEAST/FUNGI

Candida albicans (ATCC 10231)

Aspergillus niger (ATCC 6275)

Penicillium funiculosum (ATCC 9644)

Chaetomium globosum (ATCC 6205*)


*Extremely resistant strains


TESTING SPECIFICATIONS:

Bacteria and Candida

Tryptic Soy Broth used as medium grown <7 days at 32-35'C

culture started from 1:500 dilution of 18-24 hr growth


FUNGI

Sabouraud Dextrose agar used as medium grown < 28 days at 20-22'C

culture started from 1:5 dilution of fresh spore (hyphae-free) suspension from a 10 day growth

Heavy test inoculum used to enhance failure



For In Vitro Research or Further Manufacturing Use Only-all characetristics are for illustrative purposes only. Suitablity for use is the sole responsibility of the end user.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

Email:antibodies@fitzgerald-fii.com

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