NOTE: 10% Discount on purchases of 3 or more any combination of CD monoclonal antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

CD106 Antibodies

Mouse anti-human CD106 antibodies

-2 clones

CATALOG# CLONE# Workshop Host Form Price

RDI-CD106abm-B1 1G11B1 V mIgG1 purified $312.00

RDI-CD106abm-BFT " " " " FITC $375.00

RDI-CD106abm-BPE " " " " PE $500.00

RDI-CD106abm-BBT " " " " Biotin $375.00

RDI-CBL509 1.4C3 ++ -- mIgG1 purified $375.00

RDI-CBL509BT " -- " BIOTIN $375.00

++ stains formalin fixed parrafin embedded tissues

MOUSE ANTI-HUMAN CD106/VCAM-1 MONOCLONAL ANTIBODY

cat# RDI-CD106abm-B1 $312.00

Presentation: 100ug in 0.1ml (1mg/ml) 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, .5 mg/ml Gentamicin Sulfate (as a preservative).

Clone: 1.G11B1

PRODUCTION: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.

Isotype: Mouse IgG1

Immunogen: Human endothelial cells

INFORMATION: Human CD106 is an endothelial adhesion molecule that binds to a4b1 & a4b7 integrins(VLA-4) and promotes adhesion of lymphocytes, monocytes, eosinophils and basophils. CD106 is also found on follicular dendritic cells, interdigitating recticulum cells and Kupffer cells. Antibody 1.G11B1 recognizes the VCAM-1 molecule (CD106) and blocks leukocyte adhesion.

References: M.H. Thornhill, D.O. Haskard, (1991) J Immunol 146: 592-598. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1764-1767. M. Nagata, et al, (1995) J Immunol 155: 2194-2202. H.E. Chuluyan, et al, (1995) J Immunol 155: 3135-314.

STORAGE: Store at 2 - 5oC. Freeze/Thawing is not recommended.

PERFORMANCE: Cultured human umbilical cord vein endothelial cells were stimulated 4 hours with 10 ng/ml Phorbol 12-Myristate 13-Acetate. Cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD106 antibody at 5 mg/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC at a 1:60 dilution factor, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 0.89 log10 fluorescent units when compared to a Mouse IgG1 negative control (Catalog#RDI-MIGG1X1, $188.00) at a similar concentration.

Also available:

non conjugated cat#RDI-CD106abm-B1 $312.00/100ug

FITC conjugated: cat#RDI-CD106abm-BFT $375.00/120T

Biotin conjugated: cat#RDI-CD106abm-BBT $375.00/100ug

PE conjugated: cat#RDI-CD106abm-BPE $500.00/100T

For In Vitro Research Use Only

PRODUCT CODE RDI- CBL 509 $375.00/vial

also avalable:RDI-CBL509BT $375.00/100T vial Biotin labeled

CLONE 1.4C3

ISOTYPE IgG1

SOURCE Tissue culture supernatant

PURIFICATION METHOD Protein A affinity chromatography

SPECIFICITY: The antibody is specific to human vascular cell adhesion molecule (VCAM-1, 110 kDa) whose ligand is VLA-4 (CD49d/CD29, a-4/b-1 integrin subunits). The antigen is distributed across non leucocyte and leucocyte cells e.g. neural crest and fibroblasts, B cells, T cells, monocytes and large granular lymphocytes

APPLICATIONS

*This antibody works both in ELISA assays and in immunohistological sections

*The antibody has applications in studies of cell-cell interactions

*The antibody inhibits cellular adhesion

*Western blotting

*Studies of CD106 in paraffin embedded formalin fixed tissues following high temperature unmasking

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.