rev:   October 19, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


Recombinant Human Fibroblast Growth Factor-20

-Fibroblast Growth Factor-20 (FGF-20) :The FGF family plays important roles in morphogenesis, cellular differentiation, angiogenesis, tissue remodeling, inflammation and oncogenesis. Fibroblast Growth Factor-20 (FGF-20) is a newly discovered neurotrophic factor belonging to the FGF family. FGF-20 is most similar to FGF-9 and FGF-16. This recombinant Human FGF-20 is a 23.2 kDa protein containing 209 amino acid residues.

Catalog#: RDI-1041

Package Size: 15 micrograms $231.00        $219.00/package for 2 or more

                    MINI-PACK cat#RDI-1041p $94.00/3ug

                        -inquire for custom sizes/bulk packaging

Supplied: sterile filtered and the the protein was lyophilized from 20mM Tris, pH7.6 & 1.5M NaCl (no additives)

Source: E. Coli

Purity: >97% by SDS-Page and HPLC analyses. Endotoxin level is less than 0.1ng per ug (1EU/ug) of FGF-20.

Reconstitution: The lyophilized FGF-20 should be reconstituted in 20mM TRIS, pH7.6 or DI water to a concentration of 0.1-1.0mg/ml. Allow to set at least 30 minutes at 4 DEG C, tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into water or other buffered solutions and stored at 4 DEG C for up to 1 week or stored at - 20DEG C for future use.

Note:If this material is not to be used for coating or labeling(or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain concentration greater than 10ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C. Reconstituted human FGF-20 should be stored in working aliquots at -20 DEG C. AVOID FREQUENT FREEZE THAW CYCLES. EACh FREEZE THAW CYCLE WILL DIMINISH ACTIVITY.

Reactivity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is < 10 ng/ml, corresponding to a specific activity of > 1 x 105 units/mg

AA Sequence :

PLAEVGGFLG GLEGLGQQVG SHFLLPPAGE RPPLLGERRS AAERSARGGP

GAAQLAHLHG ILRRRQLYCR TGFHLQILPD GSVQGTRQDH SLFGILEFIS

VAVGLVSIRG VDSGLYLGMN DKGELYGSEK LTSECIFREQ FEENWYNTYS

SNIYKHGDTG RRYFVALNKD GTPRDGARSK RHQKFTHFLP RPVDPERVPE

LYKDLLMYT

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


RDI Division of Fitzgerald Industries Intl

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Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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