RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All antibodies are for research use only.
see below for 3 monoclonals to Chromogranin A and polyclonals
NEW! recombinant human Chromogranin A Protein (for use as std in WB or Elisa)
cat#RDI-CHRMGA-AG $469.00/5ug
cat#RDI-CHRMGA-AG $750.00/10ug
-presented in PBS buffer with 0.2% BSA and 0.09% sodium azide
-recombinat protein encoding aa 260-454 (E coli expressed)
-approx 47kda
RDI-CHRMGAbm-COC $500.00
-cocktail of clone LK2H10 and PHE5
-200ug in 1ml with BSa and 0.1% sodium azide
-ideal for histochemistry (see clone particulars below)
Mouse anti-Chromogranin A clone LK2H10
cat# RDI-CHRMGA2abm $375.00/100ug
RDI-CHRMGA2abm-1 $562.00/200ug
RDI-CHRMGA2abmX $625.00/200ug 0.2mg in 0.2ml (NO BSA/NO AZIDE)
RDI-CHRMGA2abm-BT $625.00/200ug Biotin labeled
Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml
Clone: LK2H10
Isotype: IgG1
Immunogen: Human pheochromocytoma
Fusion Partner: BALB/c splenocyte and mouse Myeloma p3-NS1-AG4-1 cells
Uses: -histochemistry (frozen and parafin sections) 1:200 for 60 min at room temp. Titer for Optimum use -it is recommended , although not required for paraffin sections to use high temp antigen release with boiling 10mM citrate buffer pH 6.0 for 1-20 min followed by cooling at room temp for 20 min.
-western blot
Positive Control: adrenal gland, normal bowel, or normal thyroid
Localization: finely granular Cytoplasmic
Mapping: recognizes a protein of 68-75kDa, identified as chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14). Although not precisely mapped, experimnetal data shows a different eiptope is seen with clone LK2H10 than clone PHE5.
Species Reactivity: human, monkey and pig. Not with sheep, rabbit, mouse, rat, guinea pig. Others not tested.-some investigators have reported rabbit and rat reactivity (must be tested independently for each application).
ref: Science 1983, 222:628-30.
Am. J. Pathol. 115, 458 (1984)
Storage: 2-8 deg c
For Research Use Only
Mouse anti-Chromogranin A clone PHE5
cat# RDI-CHRMGAPabm $375.00/100ug
RDI-CHRMGAPabm-1 $562.00/200ug
RDI-CHRMGAPabmX $594.00/0.2mg in 0.2ml (No BSA/NO AZIDE) Larger sizes quoted on request
Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml
Clone: PHE5
Isotype: IgG1
Immunogen: Human pheochromocytoma
Fusion Partner: BALB/c splenocyte and mouse Myeloma p3-NS1-AG4-1 cells
Uses: -histochemistry (frozen and parafin sections) 1:200 for 60 min at room temp. Titer for Optimum use -it is recommended , although not required for paraffin sections to use high temp antigen release with boiling 10mM citrate buffer pH 6.0 for 1-20 min followed by cooling at room temp for 20 min.
-western blot 1:500-1:1000
Positive Control: adrenal gland, normal bowel, or normal thyroid
Localization: finely granular Cytoplasmic
Mapping: recognizes a protein of 68-75kDa, identified as chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14). Although not precisely mapped, experimental data shows a different epitope is seen with clone LK2H10 than clone PHE5.
Species Reactivity: human and monkey. Not with rat and mouse. Others not tested.
ref: Ridell K, et al Lab Invest 56:64A, 1987
Am. J. Pathol. 115, 458 (1984)
Storage: 2-8 deg c
For Research Use Only
Additional Chromogranin A references
REFERENCES: Lloyd, R.V. & Wilson, B.S. Science 222, 628 - 30 (1983)
Aguirre, P., Scullx, R.E., Wolfe,H.J., and DeLellis R.A.: Argyrophil cells in Brenner tumors: Histochemical and immuno-histochemical analyses Int. J. Gynecol. Pathol. 5, 223 (1986)
Bussolati, G., Papotti, M., Sapino, A., Gugliotta, P.,Ghiringhello, B, and Azzoparadi, J.G.: Endocrine markers in argyrophilic carcinomas of the breast Am J. Surg. Pathol. 11, 248. (1987)
DeStephano, D. B., Lloyd, R.V., Pike, A.M. and Wilson, B. S.: An immunohistochemical study of hormone production and chromogranin localization Am. J. Pathol. 116, 464.(1984)
Harlow, E. et al. Antibodies: A laboratory manual, p. 359, Cold Spring Laboratory, New York, (1988)
Hearn, S. A.: Electron microscopic localization of chromogranin A in osmium-fixed neuroendocrine cells with a protein A-gold technique J. Histochem. Cytochem. 35, 795 (1987).
Lloyd, R. V., and Wilson, B. S.: Specific endocrine tissue marker defined by a monoclonal antibody science 222, 628 (1983).
McNutt, M. A., M.D. and John, W. Bolen: Adenomatous tumor of the middle ear, An ultrastructural and immunocytochemical Study Am. J. Clin. Pathol. 84, 541 (1985)
Pietroletti, R., Bishop, A.E., Carlei, F., Bonamico, M., Lloyd R.V., Wilson, B.S., Ceccamea, A., Lezoche, E., Speranza, V., and Polark, J. M.: Gut endocrine cell population in coeliac disease estimated by immunocytochemistry using a monoclonal antibody to chromogranin Gut 27, 838 (1986).
Wiedenmann, B., Waldherr, R., Buhr H., Hille, A., Rosa, P., and Huttner, W.B. : Identification of gastroenteropancreatic neuroendocrine cells in Normal and neoplastic human tissue with antibodies against synaptophysin, chromogranin A, Secretogranin I (Chromogranin B), and Secretogranin II Gastroenterology 95, 1364 (1988)
Wilson, B.S., Lloyd, R. V. et al.: Detection of chromogranin in neuroendocrine cells with a monoclonal antibody Am. J. Pathol. 115, 458 (1984) .
Wilson, B.S., Phan, S. H., and Lloyd, R.V.: Chromogranin from normal human adrenal glands: purification by monoclonal antibody affinity chromatopraphy and partial N-terminal amino acid sequence Regulatory Peptides, 13, 207 (1986)
Rabbit (monolconal) Anti-chromogranin A
-cat#RDI-CHRMG-SP12 $375.00/0.5ml
-cat#RDI-CHRMG-SP121 $625.00/1ml
-clone SP12
-rabbit monoclonal
-supernatant with 0.09% sodium azide
-reactive with human, monkey, pig, rat, and rabbit
-excellent for formalin fixed paraffin sections
-cytoplasmic staining
-positive contro: pancreas
rabbit anti-Chromogranin A RDI-CHRMGAabrX
$500.00/200ug in 1ml PBS with 0.2% BSA and 0.09% sodium azide
-reacts with human, monkey, rat, pig and rabbit (other not tested)
-made with recombinant human chromogranin A
-sutiable for formalin fixed paraffin sections (no special pretreatemnt required)
Rabbit anti- Chromogranin A+B
cat#RDI-PRO11422 $375.00/vial
Category: Rabbit polyclonal
Form/Purification Rabbit serum
Immunogen: Human pheochromocytoma extract
Clinical Application/ Marker for neuroendocrine, neuronal and Specificity adrenal tumors. In immunohistochemistry, the antiserum gives a specific reaction in pituitary antrum, duodenum, small intestine and pancreas
Recommended for Positive Control: Pancreas, duodenum
Application Immunohistochemistry Suitable for frozen and paraffin-embedded* tissue
*no proteolytic treatment required
Antigen Recognized Human, mouse, rat, rabbit
Working Dilution 1:50 - 1:100, optimal dilution should be tested by serial dilution
Dilution Buffer PBS with 0.1% Na-Azide
Incubation Time 1 h at room temperature
Storage At 4°C (undiluted) or at -20°C (aliquots)
Volume 0.25 ml.