rev:    December 17, 2003

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!


DATA SHEET:Human Cardiac Troponin I COMPLEX

see also Tropoinin Data Pack for more information and matching antibodies-

NEW PRODUCT!!!

-suitable for use as a standard in immunoassay for early detection of MI of cardiac troponin I. This material is stable after dilution in human serum.

Catalog#: RDI-CTROPICOM  or RDI-TRK8T62

DATA SHEET:Human Cardiac Troponin I COMPLEX

-suitable for use as a standard in immunoassay for  early detection of MI of cardiac troponin I.

Package Size: 100 micrograms $1250.00

                       500 micrograms $2500.00

                       1 milligram        $3500.00

                         Larger sizes quoted on Request

Supplied: 1mg in 1ml (in solution of 150mM NaCl, 5mM CaCl2, 20mM Tris-HCL, pH 7.5. -concentration of cardiac troponin I (see batch sheet below)

Concentration was determined by gel- scanning (Enhanced Laser Densitometer).

Analysis: contains human cardiac troponin I, human cardiac troponin C and human cardiac troponin T.

sample Batch Data: :

lot#03/01-8T62          Troponin I (1mg/ml), Troponin T (0.88mg/ml)

exp:Jan 2005               Troponin C (0.91mg/ml)

Ratio of the components in complex I/T/C- 1/0.61/1.18   (determined by gel scanning (enhanced laser densitometry)

Purification: The purification was performed under mild conditions without Urea treatment. Troponin complex is native and corresponds to the presentation of troponin complex in serum of AMI patients. It also shows higher stability (after dilution in human serum) in comparison with purifed cardiac troponin I.

Source: Human Heart tissue. Blood samples from the tissue donors were tested and found negative for HBsAG, HIV-1 and HIV-2 antibodies & HCV and syphillis.

Storage: -70 DEG C in liquid form. Avoid frequent freeze thaw cycles. Strongly recommend aliquoting the product.

-Avoid repeated freezing and thawing. It is recommended to aliquot the product (which is supplied in 150mM NaCl, 5mM CaCl2, 20mM Tris pH7.5) after first thawing. Store at -70 DEG C.

For better stability it is recommended to prepare stock solutions of troponin complex in normal human serum with final concentration of cardiac troponion I not less than 1000ng/ml. Store at -70 DEG C.

Precautions: For In vitro research Use Only. Not   for use in or on humans or animals or   for diagnostics . All material should be   handled at Biosafety Level II conditions   and treated as if capable of   transmitting disease.

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

this material is also part of the AACC cTNI standardization reference material

http://www.aacc.org/cln/ctnl.pdf

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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