rev: August 14, 2002

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Immunoasaay Enhancers

for Researchers and Manufacturers


COATING STABILIZERS

-helps keep what you coated active! (distributed by RDI).


cat# #RDI-PRSVRCT-1L         1000ml          $375.00  

        $344.00 2-9 Liters  $312.00/liter 10-19        $250.00/liter 20+

also available as 5X concentrate

cat#RDI-PRSVRCT-1L5X $1625.00/1Liter (5 Liter equivalent)



Preserv-a-Coat Immunoassay Stabilizer is a stabilizing solution appropriate for use with many types of immunoassay formats. The Proprietary formulation employs unique, nontoxic chemistry which maintains antibody, enzyme, or antigen activity of dried immunoassay components. The solution is ready-to-use and easy to evalate. When properly applied, Preserv-a-Coat Immunoassay Stabilizer increases the active shelf life and/or allows storage at elevated temperatures of immunoassay components. Preserv-a-Coat can replace the blocking step used in most immunoassays.


Preserv-a-Coat can be readily incorporated into most immunoassay production protocols. Simply immobilize or absorb the primary biomolecule (antigen or antibody) according to the procedure already optimized in your laboratory and wash to remove excess or weakly bound material. Immediately after washing, before the substrate has dried, add the Preserv-a-Coat solution to the coated assay substrate. Incubate for 15-45 minutes at room temperature to allow interaction of the Preserv-a-Coat ingredients with the active protein. Aspirate or drain, and dry the stabilized substrate for long term storage. Most users find that optimum stability is maintained when the final product is packaged in an airtight container with desiccant.


Preserv-a-Coat can also be incorporated into lyophilized components to increase shelf life and allow storage at room temperature.


KEY BENEFITS

-Users report the following benefits of Preserv-a-Coat Immunoassay Stabilizer:

- Easy to use without major changes in current production protocols.

Extended shelf life under all temperature conditions allowing:

' larger production runs-cost savings

' convenience of higher temperature storage

' reduction of expired product complaints from customers

Elimination of the standard blocking step. (blovking may still be required depending upon your assay)


Potential Uses:

Preserv-a-Coat is effective with a variety of EIA and RIA substrates and components:

' hydrophobic surfaces such as:

' polystyrene microwell plates or strips

' polystyrene tubes

' polymeric membrane or "dipstick" materials

' hydrophilic surfaces such as cellulose

' polyclonal or monoclonal antibodies

' antibody or antigen-enzyme conjugates


Suggested Starting PROCEDURES:

Store at 4'C. Ready to use--no dilution necessary. No preservatives added. If you do not plan to use within 7 days after opening, use aseptic technique when removing contents, or add a preservative compatible with your assay.

TO STABILIZE ADSORBED OR IMMOBILIZED PROTEINS ON MICROWELL PLATES/STRIPS

1. Immobilize or adsorb the primary protein (antibody or antigen) according to the procedure optimized in your laboratory. Wash adequately to remove excess or weakly bound protein.

2. Immediately after washing, add enough Preserv-a-Coat solution to allow interaction with the entire protein-coated surface. For example, if you added 100ul/well of the primary protein solution in step one, then the amount of Preserv-a-Coat solution you should add is also 100 ul/well. Do not let coated components dry before adding Preserv-a-Coat solution since drying contributes to the loss of protein activity.
Note: In certain assays, it may be preferable to block the plate first using 300ul per well (or full well volume, washing and then adding the 100ul of the Preserv-a-Coat.

3. Incubate for 15 to 60 minutes at room temperature. For most assays, Preserv-a-Coat can replace the blocking solution. However, if your assay demands more blocking, try mixing Preserv- a-Coat 1:1 with your current blocking solution for added blocking capability.

4. Remove or aspirate the Preserv-a-Coat solution, but do not wash.

5. Dry components for long-term storage. Products coated with Preserv-a-Coat may require longer drying times than those without Preserv-a-Coat .

Recommended methods are:

' place plates in a humidity controlled chamber (less than 15% humidity) until dry, 4-24 hours,

' or dry plates at 30'C to 40'C in a vacuum oven for 4 hours.

Drying times should be optimized for each application.

6. Package the final, stabilized product in an airtight container with a desiccant. This is especially important when the final product is stored in a humid environment or refrigerated (where condensation is likely to occur).


TO STABILIZE ADSORBED OR IMMOBILIZED PROTEINS ON MEMBRANES

1. Immobilize or adsorb the primary protein (antibody or antigen) according to the procedure optimized in your laboratory.

2. Dilute Preserv-a-Coat 1:1 in phosphate buffered saline (PBS).

3. Add just enough Preserv-a-Coat/PBS solution to coat the membrane.

4. Dry thoroughly. Preserv-a-Coat-treated membranes will require longer drying times than those without Preserv-a-Coat.

5. Package the final, stabilized product in an airtight container with a desiccant. This is especially important when the final product is stored in a humid environment or refrigerated (where condensation is likely to occur).


TO STABILIZE CONJUGATES IN DRY FORM

Following are recommended guidelines for stabilizing conjugates in a dried form:

' If you dilute your conjugate before drying/lyophilizing, use Preserv-a-Coat as the diluent. Otherwise, add between 5 and 10 parts Preserv-a-Coat to 1 part conjugate. (optimum ratio should be empirically determined). Mix gently.

' Note: If you are lyophilizing in vials with rubber stoppers, stability can sometimes be improved by placing the rubber stoppers in a 100'C vacuum oven for one hour before using them. This dries them and drives off any volatiles present in the stopper.

' Freeze the conjugate/stabilizer mixture, then lyophilize as normal. Lyophilization may require extra time due to the nature of Preserv-a-Coat or if the addition of Preserv-a-Coat results in increased volume.

' For evaporative drying, place the conjugate/stabilizer mixture in 37-40'C oven for 4 hours or until completely dry. The volume per container should be low enough to allow maximum surface area to be exposed to air during drying. Store the final product in an airtight container.


For In Vitro Research or Further Manufacturing Use Only

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

Email:antibodies@fitzgerald-fii.com

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