rev: May 8, 2007

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Biogenic Amine Assays for Pharmaceutical and Specialty Research

-summary product data: please request full insert for current and more detailed information

The following Elisa kits are distributed by RDI Divison of Fitzgerald Industries Intl for in vitro research use only-not for use in or on humans or animals, not for use in diagnostics.

Metanephrine ELISA  cat#RDI-RE59181

enzyme immunoassay for the quantitative determination of metanephrine in urine.

Clinical Significance:

The catecholamines epinephrine, norepinephrine and dopamine are synthesized in the adrenal medulla, the sympathetic nervous system and in the brain. As catecholamines and their metabolites metanephrine are secreted in increasing amounts in a number of diseases, they may be used for diagnostic purposes.

In this context, diagnosis as well as the followup of tumor diseases of the nervous system are of special importance. This applies primarily to the pheochromocytoma, but also the neuroblastoma and the ganglioneuroma. Malignant gowth is described in 10% of pheochromocytomas. Furthermore, an increase of catecholamines and their metabolites metanephrine and normetanephrine can be observed in the carcinoid.

Principle of Assay:

The AMICYL-Test (tm) Metanephrine-Elisa kit provides materials for the quantitative measurement of chemically derivatized metanephrine in urine. The sample preparation, derivatization of metanephrine to N-acylmetmetanephrine, is part of the sample dilution and is achieved by incubation of the respective sample with the "Acylation Reagent".

The assay procedure follows the basic principle of competitive ELISA: The competition between the amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is acheived by comparing the enzymatic activity of unkown with a response curve prepared by using known standards.

Assay Characteristics:

reference values for 24 hour urine( each lab must establish its own reference range).

  metanephrine:  20-345 ug/d

Sensitivity: approx  1-ug/l urine (3 S.D. of zero standard).

Specificity:

Compound % cross reactivity
Metanephrine 100.00
epinephrine     0.5
Normetanephrine     0.3
4-hydroxy-3-methoxy

phenylglykole

    0.01
3-methoxytyramine    <0.01
norepinephrine   <0.01
Dopamine   <0.01
L-Dopa   <0.01
4-Hydroxy-3-methoxy

-phenylpyruvat acid

  <0.01
ferula acid   <0.01
vanillic mandelic acid  <0.01
L-Tyrosine  <0.01
DL-Tryptophane  <0.01
Caffeic acid  <0..01
Ferula acid  <0.01
Homovanillic acid  <0.01

Recovery: 74-120% (range tested 1529 to 47 ug/l)


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RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (201) 584-7093

fax      (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com