rev:   March 10, 2004

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of monoclonal CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD95 (FAS ) & Fas-L  (CD95L) Antibodies


Mouse anti-human CD95 (Fas) and FAS-Ligand antibodies

-5 clones   (CD95)    2 for  CD95L (Fas-L)


see also new recombinant Fas antigen (intact, extracellular and intracellular fragments)

CATALOG#                 CLONE#                  Workshop    Host         Form        Price 

RDI-CBL527BT              "                                  "                 mIgG1      Biotin        $375.00

RDI-CBL537                B-D29                           VI               mIgG1      purified     $375.00

RDI-M1714clb             FAS18                                            mIgG2a    purified    $375.00

RDI-M1715 clb            FAS19                                         mIgG1      purified    $375.00

RDI-M1735 clb            FAS19                                         mIgG1      FITC        $375.00

RDI-FASCD95abm          -----                           ---           mIgG1      purified     $ see below

RDI-CD95-DX2              DX2                           ---            mIgG1      purified     $375.00

RDI-CD950DX2-BT         ""                               ---             "              Biotin       $469.00

RDI-FASLabm                 -----                           ---           mIgG1      purified     $ see below

RDI-CD95L-NOK1        NOK1                                        mIgG1      purified     $438.00


Mouse anti-HUMAN Anti-CD95


PRODUCT CODE RDI-CBL527     $375.00/vial 200ug

   also available Biotin labeled cat#RDI-CBL527Bt   $375.00/100T vial

CLONE: B-G27

ISOTYPE: IgG1

SOURCE: tissue culture supernatant

PURIFICATION Protein A affinity chromatography

SPECIFICITY: The CD95 antigen, molecular weight 40-50kDa, is a cell surface glycoprotein that was originally identified by two apoptosis inducing antibodies, anti-APO-1 and anti-FAS. FAS is known to induce signal into cells ad anti-FAs antibody works as an agonist for the FAS protein. CD95 is expressed on a minority of resting T and B cells and monocytes whose viability is unaffected by anti-CD95 antibodies. In marked contrast anti-CD95 antibodies induce apoptosis in activated T and B cells, NK cells and thymocytes.

APPLICATIONS:

*Flow cytometric studies

*Studies of apoptosis without immobilization of the antibody, using the EBV cell line SKW69 0.25ug/ml of CBL527 will induce 50% apoptosis in a 24 hour bioassay.


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in in phosphate buffred saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 30 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures


Bulk material, no BSA and/or no azide available upon request.

Order cat#RDI-CBL527-1XP $1062.00/milligram Larger quantities quoted upon request.


MOUSE MONOCLONAL  ANTIBODY TO: HUMAN CD95

PRODUCT CODE  RDI-CBL 537

CLONE  B-D29

ISOTYPE  IgG1

IMMUNIZING ANTIGEN  Recombinant Fas antigen

FUSION PARTNER X63 Ag 8653 myeloma cell line

SOURCE  Tissue culture supernatent

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY : The CD95 antigen, molecular weight 40-50kDa, is a cell surface glycoprotein that was originally identified by two apoptosis- inducing antibodies, anti-APO-1 and anti- Fas. Fas is known to induce an apoptotic signal into cells and anti-Fas antibody works as an agonist for the Fas protein. CD95 is expressed on a minority of resting T and B cells and monocytes whose viability is unaffected by anti-CD95 antibodies. In marked contrast anti-CD95 antibodies induce apoptosis in activated T and B cells, NK cells and thymocytes.

APPLICATIONS

*Flow cytometric studies of the CD95 antigen

*This antibody will block the CD95 antigen

The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

-bulk material on request


cat#RDI-M1714clb  mouse anti-human CD95  200ug in  1ml with 10mg BSA in 20mM Tris and 150mM NaCl and                                            0.001% merthiolate.  mIgG2a  clone FAS 18 (induces apotosis)   $375.00/vial

-also available FITC labeled cat#RDI-M1734clb  $375.00/100 Tests


cat#RDI-M1715clb  mouse anti-human CD95 200ug in 1ml with 10mg BSA in 20mM Tris and 150mM NaCl and 0.001%                                 merthiolate. mIgG1   clone FAS19 (inhibits induction of apotosis by clone FAS18).

-also available FITC labeled cat#RDI-M1735clb  $375.00/100 tests vial



Mouse anti-FAS Monoclonal ab

cat# RDI-FAS95abm        $412.00/vial 50ug

        RDI-FAS95abm-1    $750.00/vial 150ug

Background: Fas (also known as Apo-1 and CD95) is a member of a family of cell surface receptors which includes TNFR, NGFR, CD40, CD27, CD30 and 4-1BB. Both the murine and human Fas genes have been cloned and share 60% similarity in their deduced amino acid sequences. Fas is expressed in the thymus, liver, heart, and ovary. Fas appears to be a cell surface apotosis signalling molecule which is expressed in a number of different cell types. Abnormalities in the Fas gene are correlated with autoimmune features in mice and unusually high levels of lymphocyte apotosis in HIV infected humans. Genetic studies localized the Fas gene near lpr (lymphoproliferation disease) locus on mouse chromosome 19. Further characterization revealed that lpr is a mutation affecting function of the Fas gene. recent cloning of the Fas ligand (FASL) revealed that it is a member of the TNF family. It has been postulated that Fas plays a role in T cell mediated cytotoxicity and possibly in the normal apoptotic process that occurs during T cell development.

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: an 18.2 kDa protein fragment corresponding to amino acids 1-163 of human Fas

USe: Suitable for western blot on human, rat, mouse and chicken under using approx 0.2 to 0.05ug/ml ab on total cell lysate of human Jurkat cell. Major band at Mw 45 kDa. Also suitable for immunofluorescence.

Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.


Main REF:

-Hanabuchi, S. et al, (1994) Proc. Natl. Acad. Sci USA 91:4930.

-Takahashi, T. et al. (1994) Cell 76:969

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


DATA SHEET: mouse anti-human CD95 clone DX2 (HISTOCHEMISTRY)


Catalog#: RDI-CD95-DX2 $375.00/vial

also available: biotin labeled cat#RDI-CD95-DX2BT $469.00/100 tests

cat#RDI-CD95-DX21X   $875.00/0.5mg (no azide, o,2micron filtered)
FITC and BULK (no azide upon request)


Package Size: 100ug purified in 50mM Tris-Hcl (pH 8.0), 0.15M NaCl and 0.1% sodium azide. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)

Prep: purified from tissue culture supernatant via Protein G affinity chromatography.


Species: mouse IgG1k


CLONE: DX2


Workshop Code: N/A


Activity: Reacts with the 45kD transmembrane cell surface FAS or APO-1 antigen, designated CD95. CD95 is expressed on a variety of normal and neoplastic cells, and is a polypeptide which plays a role in the programmed sequence of events leading to cell death (apoptosis). the DX2 clone specifically reacts with murine L cells, murine L1210 leukemia cells and murine P815 mastocytoma cells transfected with human Fas cDNA but not with untransfected parental cell lines. Cross linking with DX2 delivers an apoptotic signal indicating that DX2 recognizes a functional epitope of CD95 antigen.


Application: -Histochemistry for staining acetone fixed, frozen tissue sections.

-flow cytometry-Titer for each application



References:
1) Schlossman, S. et al, ed.s 1995, Leucocyte Typing V, White Cell Differentiation Antigens, Oxford University Press, NY.

2) J. Exp. Med, 177:1547

3) Cell 66:233


Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


DATA SHEET: mouse anti-human CD95L clone NOK-1


Catalog#: RDI-CD95L-NOK1  $438.00/vial

also available: biotin labeled  cat#RDI-CD95L-NKBT   $531.00/100 tests

No azide form  cat#RDI-CD95L-NKX $750.00/250ug


Package Size: 100ug purified in 50mM Tris-Hcl (pH 8.0), 0.15M NaCl and 0.1% sodium azide. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)


Prep: purified from tissue culture supernatant via Protein G affinity chromatography.

Species: mouse IgG1

CLONE: NOK-1

Activity: Reacts with Fas Ligand (FAS-L), a 40 KD type II integral membrane protein, homologous with tumor necrosis factor (TNF) and expressed on activated T and NK cells.HUman FASL is released from a 40Kd molecule as a 26KD soluble protein from transfectant cells or activated T cells by matrix metalloproteinases (MMPs). FASL mediates FAS+ target-cell lysis by activated T and NK cells through FAS/FAS/L interaction.. This mechanism has been implicated in the pathogenesis of autoimmune disorders and viral infections.


Application: -flow cytometry

                   -ELISA

                    -functional inhibition assays

                    -immunoprecipitation


References:
1) Proc Natl Acad Sci USA 91:4930

2) J. Immunol 154:3806

3) J. Exp Med 182:1777


Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


DATA SHEET: mouse anti-MOUSE CD95L FAS-L clone K10

Catalog#:     RDI-MCD95L-K10      $531.00/vial

also available: PE labeled cat#RDI-MCD95L-K10PE $625.00/200ug


Package Size: -500ug purified in 50mM Tris-Hcl (pH 8.0), 0.15M NaCl and 0.1% sodium azide.

                      -200ug conjugated in 10mM PBS pH 7.2 with 0.1% NaN3 (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)


Prep: purified from tissue culture supernatant via affinity chromatography.

Species: mouse IgG2b (mouse type C57BL/6 gld/gld)


CLONE: K10 also referenced as KAY-10


Activity: Reacts with Fas-Ligand expressed on activated T lymphocytes of selected strains of mice (C57BL/6, C3H, MRL, NOD, NZB, NZW and SJL). It does not react with similarly activated T blasts from BALB/c, DBA/1 or DBA/2 mice. It also reacts with COS cells transfected with mFASL cDNA derived from C57BL/6 and C3H mice, but not with mFASL cDNA from BALB/c nor DBA/2 mice. In addition, this clone efficiently blocks cytotoxic activity of a C3H T cell line but not a BALB/c T cell line. Phenotypic and genotypic characterizations of mouse FAS-Ligand reveal the existence on activated T cell lines and in spleen, testis and eye. FASL mRNA has been demonstrated at various levels in bone marrow, thymus, spleen, lymph node, lung, small intestine, testis and uterus.

Morever, T cell activators but not B-cell activators enhanced the expression of FasL mRNA in splenocytes; and FasL mRNA was restricted to the T cell lineage among a panel of cell lines from lymphoid tissues. FasL is not functional in mice homozygous for the gld (generalized lymphoproliferative disease) mutation; these mice cannot limit the expansion of activated lymphocytes and develop autoimmune disease. FasL is a type II membrane protein a member of the TNF/NGF family, which binds to CD95 (FAS). FasL and its counter-receptor CD95 are believed to participate in T cell development, the regulation of immune responses, and cell mediated cytotoxic mechanisms. Functional studies suggest that mFasL.2 has higher specific activity than mFasL1. It has been demonstrated that human FASL is released from the surface of transfectant cells and activated human T cells by metalloproteinases.


Application: -immunofluorescent staining in the presence of  metalloproteinase inhibitor on mFAS-L transfected and activated T cell lines (1-4ug/million cells). NOTE:PE has   been reported to bind to mouse B cells via FCgII receptors. For   immunostaining of mouse cells, this can be prevented by   preincubating cells with a CD16/CD32 antibody.


References: 1) Kayagaki, N. et al, 1997: Proc. Natl Acad Sci USA in press

                   2) J. Exp Med 182:1777-1783.


Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


DATA SHEET:      Hamster anti-MOUSE CD95L FAS-L   clone MFL3

Catalog#: RDI-MCD95L-FL3    $500.00/vial

Package Size: -500ug purified in 50mM Tris-Hcl (pH 8.0), 0.15M NaCl and 0.1% sodium azide.


Prep: purified from tissue culture supernatant via affinity chromatography.

Species: Armenian Hamster IgG

CLONE: MFL3

Antigen: C57BL/6 mouse FasL c DNA transfected baby hamster kidney cells

Activity: Clone MFL3 reacts with mouse Fas Ligand (Fas L), a member of the TNF/NGF superfmaily. Fas L has been reported to be expressed by activated mouse T cells as well as some T-cell lines and clones. Mouse FasL mRNA has been found in spleen, thymus, lung, lymph nodes, eye and testis. Fas L is a tyep II transmembrane protein which binds to its counter- receptor CD95 (FAS) and is speculated to participate in regulation of a variety of immunological responses and lymphocyte development. Clone MFL3 has been reproted to efficiently inhibit the cytotoxicity of mouse FAS-L transfected cells against human Fas-transfected cells.

References: 1) Kayagaki, N. et al, 1997: Proc. Natl Acad Sci USA 94:3914-3919

                   2) Cell 76:959-962

                   3) Int Immunol 6:1545-1553

                   4) Nature 373:444-448

Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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