rev:   April 15, 2004

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD monoclonal antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD40 & 40L Antibodies


Mouse anti-human CD40 antibodies

-4 clones  


CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-CD40abm-BE         BE-1                            V             mIgG1        Purified

RDI-CD40abm-EA         EA-5                            V             mIgG1       Purified

RDI-CBL486BT               " "                                "                "             Biotin        $375.00

RDI-M1691clb               CLB-14G7                                   mIgM        purified      $375.00

RDI-CD40L-2431          24-31                           --             mIgG1       purified     $438.00

RDI-CD154abm-TP        Trap-1                         --             mIgG1       purified     $406.00


mouse anti-human CD40  clone BE-1

clone BE-1, mouse IgG1, recognizes the CD40 molecule of about 44kD. suitable for flow cytometry, immunoprecipiation and acetone fixed frozen sections, Binding of cross linked BE-1 will partially activate B Cells

Also available:

non conjugated  cat#RDI-CD40abm-BE         $312.00/100ug

FITC conjugated: cat#RDI-CD40abm-BEFT   $375.00/120T

Biotin conjugated: cat#RDI-CD40abm-BEBT   $375.00/100ug

PE conjugated: cat#RDI-CD40abm-BEPE        $469.00/100T

mouse anti-human CD40 clone EA-5

clone EA-5, mouse IgG1,recognizes the CD40 molecule of about 44kD. suitable for flow cytometry, immunoprecipiation and acetone fixed frozsn sections, Binding of cross linked EA-5 will partially activate B Cells

also available

non conjugated  cat#RDI-CD40abm-EA          $312.00/100ug

FITC conjugated: cat#RDI-CD40abm-EAFT  $375.00/120T

Biotin conjugated: cat#RDI-CD40abm-EABT $375.00/100ug

PE conjugated: cat#RDI-CD40abm-EAPE      $469.00/100T

 Bulk quotes on request


Mouse Anti-Human CD40 ANTIGEN

PRODUCT CODE :RDI-CBL486  $375.00/vial 200ug

also available: Fitc labeled cat#RDI-CBL486FT $375.00/100T - DISCONTINUED

                      PE labeled cat#RDI-CBL486PE $469.00/100T - DISCONTINUED


CLONE: B-B20

ISOTYPE: IgG1 kappa


SOURCE: Mouse ascitic fluid

ANTIGEN: Raji cell line

Fusion Partner: P3X63/AG.8653 myeloma cell line


PURIFICATION METHOD: Ion exchange chromatography

SPECIFICITY: The antibody reacts with CD40, a 48kDa glycoprotein which is found on all mature peripheral B lymphocytes but is absent from terminally differentiated plasma cells.. It is present on some epithelial cells, carcinomas and lymphoid dendritic cells.


APPLICATIONS:
* Quantitative determination of peripheral B cells in blood

* Identification of CD40 positive cells in tissue sections including paraffin embedded sections.

* The antibody promotes homotypic adhesion and gives a strong cycle progression signal

* The CD40 antigen is a possible receptor for a soluble product/cellular ligand supporting proliferation of B cells

* Flow cytometry


References: Clark, E.A. & Ledbetter, J.A. Proc. Natl Acad Sci 83,4 494 (1986)

                      Stamenkovic, I et al, EMBO J. 8, 1403-10 (1989)

                      Leucocyte Typing IV, Oxford University Press (1985)

                      Lederman S. J. et al, Immunol 149, 3817-26 (1992)

                      O'Grady J.T. et al. Am J. Path. 144, 21-26 (1994)

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 200ug in 2ml phosphate buffered saline solution with 1mg/ml BSA and 10mM NaN3 .We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -200C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


cat#RDI-M1691clb  clone CLB-14G7  $375.00/200ug vial  inquire for specs


Rabbit (polyclonal) anti-CD40   (Human/Mouse/Rat/Monkey)

cat# RDI-CD40abrX   $500.00/100ug  

Presentation: 100ug in 0.1ml (1mg/ml) 25 mM sodium borate, 100 mM boric acid, 75 mM NaCl, 5 mM EDTA, pH 8.2. No preservative

Immunogen: A 20 residue peptide corresponding to amino acids 258-277 of the human CD40 sequence (CQPVTQEDGKESRISVQERQ) coupled to KLH.

Use: Working dilution: 1:250 for immunoblotting (colorimetric) > with chemiluminescence. Titer for each application.

Specificity: Recognizes a 43 kDa band corresponding to the expected molecular weight of CD40 by SDS-PAGE. Reacts with CD40 in human, mouse, rat, pig, and monkey cells.

Storage: -20°C. Recommend aliquot and store at -20 DEG C. Avoid frequent freeze thaw cycles. At first thaw, tighten cap and centrifuge for 1 minute 1000rpm to concnetrate material in vial.

General References:

Stamenkovic, I.C., et al. 1989. EMBO J. 8,1403.

Grant, P.A., et al. 1996. EMBO J. 15, 6691.

Lagresle, C., et al. 1996. J. Exp. Med. 183, 1377.

Peuguet-Navarro, J., et al. 1997. J. Immunol. 158,144.

Ishida, T., et al. 1996. J. Biol. Chem. 271, 28745.

Berberich, I., et al. 1996. EMBO J. 15, 92.

For Research Use Only


DATA SHEET: mouse anti-human CD 40L (gp39) clone 24-31


Catalog#: RDI-CD40L-2431       $438.00/vial    

 
Also available conjugated to FITC: #RDI-CD40L-2431-FT $469.00/100T

also available Biotin conjugated cat#RDI-CD40L-2431BT $469.00/100ug

also available PE conjugated: cat#RDI-CD40L-2431PE $625.00/100T

Package Size: 100ug in 100ul (1mg/ml) 50mM sodium phosphate pH 7.5, 500mM Potassium Chloride, 150mM NaCl, 0.5mg/ml Gentamicin Sulfate (preservative).

Species: mouse IgG1

Antigen: human sgp 39 fusion protein

Purity: >95% immunoglobulin by SDS-PAGE. Purified from low FBS containing tissue culture supernatant using Protein A. Product contains <1% bovine immunoglobin.

LOT# see sheet with shipment


CLONE: 24-31

Activity: Reacts with human CD40L. This antibody immunoprecipitates a CD40L (gp39) molecule of 36 kd. The antibody will stain activated CD3+human PBL and will functionally block MLR, sgp30 induced B cell proliferation and T cell dependent B cell differentiation.

Application: -Indirect fluorescence (approx 0.8ug/500K cells) -Briefly, ficoll prepared human peripheral white blood cells were stimulated by incubating 6 hours at 5 million cells/ml in RPMI 10% FBS media including 3ug/ml phytohemagglutinin-P and 1ng/ml Phorbol 12- Myristate 13-acetate. 500K cells were then washed and incubated 45 minutes on ice with 80ul of product at 10ug/ml. Cells were washed 2X and incubated with 50ul of secondary antibody and then washed 3X. A net of 5% of population of the cells stained positive with a mean shift of 306 fluorescent units compared to the IgG1 negative control.

-Blocking experiments-MLR (mixed Lymphocyte Response) can be blocked using approx 10ug/ml when 300K human PBL's(responders) are cultured with 100K irradiated allogenic human PBL's (stimulators). Inhibition can be observed by day 5 when using a 3H thymidine pulse to monitor proliferation. B cell differentiation induced by activated T cells and Il-2 can be measured by Ig production of IgD+ naive B cells.

Monoclonal anti-CD40L significantly inhibits this Ig   production at levels of 1-10ug/ml.

-titers must be determined for each application

Storage: Store at 4 Deg C. DO NOT FREEZE.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

References: Dr. Randolph J. Noelle, Dartmouth Medical School


It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET: mouse anti-human CD40L (CD154) clne TRAP1

Catalog#: RDI-CD154abm-TP   $406.00/100ug

Package Size: 100ug purified in 50mM Tris-HCL (pH 8.0) with 0.01% NaN3.

       also available azide free: cat#RDI-CD154abm-TPX  $1000.00/0.5mg

Species: mouse IgG1k

Clone: TRAP-1

Workshop: VI, id#6T-068

Purification: By Protein G from supernatant.

Activity: Reacts with CD40-Ligand (newly clustered as CD154 in VIth workshop), a 39 kd type II membrane glycoprotein expressed on activated T cells. CD40-CD40L interaction plays a very important role in T cell depenednet B cell proliferation, differenetiation, and memory cell formation. CD40L MAB partially blocks T cell-B cell interaction affecting subsequent proliferation, IL-2R expression and differentiation on B cells. In addition, blocking of CD40-CD40L, interaction has been demonstrated with soluble CD40, resulting in the inhibition of immunoglobulin isotype switching. It has been reported that patients with X linked hyper-IgM syndrome have defective expression of functional CD40L due to a defective gene that encodes CD40L.


uses: -flow cytometry,

         -blocking experiments (azide free material)

ref: J. Clin nvest 93:1315

      Eur J immunol 24:787

      J. Exp Med 180:141

      Leucocyte Typing VI, Garland Press.

Storage: Store 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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