rev: March 10, 2003

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CTLA-4 Antibodies  (CD152)


Mouse anti-human CTLA-4 antibodies

-2 clones


CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-CTLA4abm             BNI3.1                          ---           mIgG2a     purified     $438.00

RDI-CTLA4-BT               ""                                 ---             "               Biotin        $500.00

RDI-CD152-H5PE           ANC152.2/8H5           --             mIgG2      PE              $594.00

see also monoclonals/polyclonals related antibodies CD28,   CD80   &   CD86


DATA SHEET: mouse anti-human CD152 (CTLA-4):PE CONJUGATED

Catalog#: RDI-CD152-H5PE or  RDI-CTLA4-PE    $594.00/vial

Package Size: 120 Tests in 200ul 50mM sodium phosphate pH 7.5, 500mM Potassium Chloride, 150mM NaCl, 15% Glycerol 0.2% BSA &0.04% NaN3.

Species: mouse IgG1

Antigen: stimulated human PBL's andhuman CD152 Ig fusion protein

Purity: Purified by protein A from tissue culture supernatant. Conjugated with PE and further purified by size exclusion column Approx 1mg/ml A565/A20 ratio 3.3.

CLONE: ANC152.2/8H5

Application: -direct fluorescence (approx 80ul of 1:50 dilution/500K cells)

-Briefly, ficoll prepared human peripheral blood lymphocytes were stimulated in culture 3 days in the presence of PHA-P (5ug/ml). 500K cells were then washed and pre-incubated 5 minutes on ice with 20ul of 250ug/ml human IgG (to block nonspecific binding) and then incubated 45 minutes on ice with 80ul of product at 1:50 dilution. Cells were washed 3X and analyzed.

Storage: Store at 4 Deg C. DO NOT FREEZE.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

recommended isotype control: RDI-MIGG1XPE $188.00/vial

also available:

non labeled cat#RDI-CD152abm-H5            $375.00/100ug

Biotin labeled cat#RDI-CD152abm-H5BT     $469.00/100ug

non labeled Fab'2 fragment cat#RDI-CD152abm-H52  $1000.00/100ug


DATA SHEET: mouse anti-human CTLA-4  (CD152)

Catalog#: RDI-CTLA4abm       $438.00/vial



Package Size: 100ug purified in 50mM Tris-Hcl (pH 8.0), 0.15M NaCl and 0.1% sodium azide.(sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)


Prep: purified by Protein G from supernatant

Species: mouse IgG2ak

CLONE: BNI3.1

Activity: Reacts with the cytolytic T lymphocyte associated antigen (CTLA-4). CTLA-4 is transiently expressed on activated CD28+ T cells and binds to CD80 and CD86 present on antigen presenting cells (APC) with high avidity. This interaction appears to deliver a negative regulatory signal to the T cell. There are recent reports which indicate that CTLA-4 is also expressed on B cells when cultured with activated T cells suggesting a possible role of CTLA-4 in the regulation of B cell response.

Application: -Indirect immunofluorescence cell surface staining , Titer for each application


References:

1) J. immunol 151:3469

2) J. immunol. 155:1776.

3) J. immunol. 156:1047.

Storage: Store at 4 DEG C..


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible forany patent infringements that might result with the use of or derivation of this product.


Also available Biotin labeled, cat#RDI-CTLA4-BT    $500.00/100T

                      no azide prep:  cat#RDI-CD152-1X     $938.00/0.5mg




-All products are for in vitro research use only-Not for use in or on humans or animals-Not for Diagnostic use. Not responsible for any patent infringements with the use or derivation of these products. Price/specifications/availability subject to change without notice.
RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222 or

          (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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