rev: March 10, 2003
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: 10% Discount on purchases of 3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Mouse anti-human CTLA-4 antibodies
CATALOG# CLONE# Workshop Host Form Price
RDI-CTLA4abm BNI3.1 --- mIgG2a purified $438.00
RDI-CTLA4-BT "" --- " Biotin $500.00
RDI-CD152-H5PE ANC152.2/8H5 -- mIgG2 PE $594.00
see also monoclonals/polyclonals related antibodies CD28, CD80 & CD86
Catalog#: RDI-CD152-H5PE or RDI-CTLA4-PE $594.00/vial
Package Size: 120 Tests in 200ul 50mM sodium phosphate pH 7.5, 500mM Potassium
Chloride, 150mM NaCl, 15% Glycerol 0.2% BSA &0.04% NaN3.
Species: mouse IgG1
Antigen: stimulated human PBL's andhuman CD152 Ig fusion protein
Purity: Purified by protein A from tissue culture supernatant. Conjugated with PE and further purified by size exclusion column Approx 1mg/ml A565/A20 ratio 3.3.
Application: -direct fluorescence (approx 80ul of 1:50 dilution/500K cells)
-Briefly, ficoll prepared human peripheral blood lymphocytes were stimulated in culture 3 days in the presence of PHA-P (5ug/ml). 500K cells were then washed and pre-incubated 5 minutes on ice with 20ul of 250ug/ml human IgG (to block nonspecific binding) and then incubated 45 minutes on ice with 80ul of product at 1:50 dilution. Cells were washed 3X and analyzed.
Storage: Store at 4 Deg C. DO NOT FREEZE.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
recommended isotype control: RDI-MIGG1XPE $188.00/vial
non labeled cat#RDI-CD152abm-H5 $375.00/100ug
Biotin labeled cat#RDI-CD152abm-H5BT $469.00/100ug
non labeled Fab'2 fragment cat#RDI-CD152abm-H52 $1000.00/100ug
DATA SHEET: mouse anti-human CTLA-4
Catalog#: RDI-CTLA4abm $438.00/vial
Package Size: 100ug purified in 50mM Tris-Hcl (pH 8.0), 0.15M NaCl and 0.1% sodium azide.(sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)
Prep: purified by Protein G from supernatant
Species: mouse IgG2ak
Activity: Reacts with the cytolytic T lymphocyte associated antigen (CTLA-4). CTLA-4 is transiently expressed on activated CD28+ T cells and binds to CD80 and CD86 present on antigen presenting cells (APC) with high avidity. This interaction appears to deliver a negative regulatory signal to the T cell. There are recent reports which indicate that CTLA-4 is also expressed on B cells when cultured with activated T cells suggesting a possible role of CTLA-4 in the regulation of B cell response.
Application: -Indirect immunofluorescence cell surface staining , Titer for each application
1) J. immunol 151:3469
2) J. immunol. 155:1776.
3) J. immunol. 156:1047.
Storage: Store at 4 DEG C..
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible forany patent infringements that might result with the use of or derivation of this product.
Also available Biotin labeled, cat#RDI-CTLA4-BT $500.00/100T
no azide prep: cat#RDI-CD152-1X $938.00/0.5mg
34 Junction Square Drive
Concord MA 01742-3049
phone (800) 370-2222 or
(978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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