RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN 8
Mab to Cytokeratin 8 cat#RDI-PRO61038 $375.00/vial of 50ug
-also available Biotin conjugated: cat#RDI-PRO61538 $438.00/0.25ml
Clone Determination Ks 8.7
Category Mouse monoclonal
Immunoglobulin Class IgG1
Purification SpAss-Protein A (recombinant)
Antigen Cytoskeletal proteins from cultured HeLa cells
Specificity Ks 8.7 represents an excellent marker to discriminate simple
epithelia from those of different origin
Application Suitable for frozen tissue and paraffin- embedded sections
after microwave treatment Cytological material Immunoblotting (Western)
Stability At 4°C one year after reconstitution
Quantity 50 µg (lyoph.)
Polypeptide Reacting MR 52 500 polypeptide (cytokeratin 8) of human epithelia
Antigen Recognized in Human, mouse, rat, tupaia belangeri, monodelphis domestica, hamster; negative with bovine
Reactivities on Cultured MCF-7, RT 112, PLC
Tumors Specifically Detected All adeno-CA tested; undifferentiated CA; cervix CA; hepatocellular CA
Working Dilution When reconstituted with 1 ml distilled water, dilute further 1:10 Incubation Time 1 h at RT
References
Bartek J., Vojtsek B., Staskova Z., Bartkova J., Kerekes, Z., Rejthar, A. and Kovraik, J.: A series of 14 new monoclonal antibodies to keratins: Characterizationi and value in diagnostic histophathology. J Pathol 164, 215-244 (1991)
Heid H., Vojtesek, B., Bruder, G., Kaufman, M., Staskova, Z., Kovarik, J. Bartek, J. and Franke, W.W.: Cell type heterogeneity of mammary gland epithelial and breast carcinoma cells as revealed by cytokeratin expression. Differentiation (1991), submitted
Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982)
Osborn, M., van Lessen, G., Weber, K., Klöppel, G. and Altmannsberger, M.: Methods in Laboratory Investigation. Differential diagnosis of gastrointestinal carconomas by using monoclonal antibodies specific for individual keratin polypeptides. Lab. Investigation 55,4, 497-504 (1986)
Gomi, H., Yokoyama, T., Fujimoto, K., Ikeda, T., Katoh, A., Itoh, T. and Itohara, S.: Mice Devoid of the Glial Fibrillary Acidic Protein Develop Normally and Are Susceptible to Scrapie Prions. Neuron, Vol. 14, 29-41 (1995)
Stumptner C et al, Hepatology 35, 1053-1062 (2002)
For research use only. Not supplied for use in human diagnostic or therapeutic procedures.