rev:   March 3, 2005

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Actinin


MOUSE MONOCLONAL ANTIBODY TO: HUMAN ACTININ

PRODUCT CODE  RDI-CBL231   $375.00

CLONE    AT 6/172

Immunogen   Humna platelet alpha actinin

ISOTYPE   IgG1

SOURCE   Tissue culture supernatant

PURIFICATION METHOD   Protein A affinity chromatography

SPECIFICITY

This antibody immunoblots with a platelet a-actinin band (100 kDa) on SDS/PAGE gels. Immunofluorescence staining on methanol fixed fibroblasts shows adhesion plaque and stress fibre areas. This antibody recognizes an epitope in the N-terminal region of alpha-actinin, probably between residues 1-24.

APPLICATIONS

* The antibody is suitable for use on frozen sections (paraffin sections after high temp antigen release pretreatment in citrate buffer pH6.0)

* Suitable for immunoblotting

*IP (reported)

KNOWN SPECIES  CROSS REACTIVITY

This antibody is known to cross-react with rabbit actinin

REF:   Porter, R.M. et al., (1993) J. Pathology. 170:435-440.

           Kubler, D.M. et al., (1991) J. Cell Sci. 100:153-165.

LOT SPECIFIC DATA

The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Also available: pure actinin protein cat#RDI-CBL62002   $438.00/250ug

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


Also available: pure actinin protein cat#RDI-CBL62002    $438.00/250ug


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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