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Androgen RECEPTOR ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat# RDI-A1RECPTabm $406.00/100ug
Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM
NaN3. (Bulk available without BSA/Azide upon request) concentration
Immunogen: synthetic peptide , aa 299-315 of human AR
sequence: S T E D T A E Y S P F
K G G Y T K
Clone: AR 441
Reactivity: human (does not react with mouse)
Uses: -Western blot: 1ug/ml
-histochemistry (frozen and paraffin sections) approx 1:100 , 60 minutes RT
(paraffin * (Staining of formalin-fixed tissues
REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min
followed by cooling at RT for 20 min.)
Background: Recognizes a protein of 110kDa which is identified as androgen receptor (AR). It reacts with full length, and the newly described A form of the receptor. It does not cross react with estrogen, progesterone, or glucocorticoid receptors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormone-responsiveness and thus it may be useful in identifying patients likely to benefit from anti- androgen
Storage: 2-8 deg c
Mol Endocrinol, 2:1265-75, 1988.
Science, 240:889-95, 1988.
Cell, 56:335-44, 1989.
Crit Rev Biochem Mol Biol, 24:101-17, 1989.
FASEB J, 5:3092-9, 1991.
FASEB J, 5:2243-9, 1991.
Clin Chem, 39(2):346-52, 1993.
For Research Use Only-Not for use in diagnostics
cat#RDI-ANDRECPTabr $438.00/100ug vial
Steroid receptors are ligand-dependent intracellular proteins that stimulate
transcription of specific genes by binding to specific DNA sequences following
activation by the appropriate hormone. Androgens induce development in
male-specific organs, such as the prostate and seminal vesicle, and later
in life they control the functional activity of target tissues. Androgens
exert their effects by binding to the androgen receptor (AR) which has been
found in a variety of tissues, including reproductive organ tissue, central
nervous system tissue, and skeletal muscle.
This antibody is known to react with numerous species including primates, bovine, sheep, pigs, rodents, birds, frogs and fish. This product does not cross-react with estrogen, progesterone, or glucocorticoid receptors. Competition studies using the antigenic peptide at a 10 molar excess, completely blocked nuclear staining whereas other non-related peptides had no effect on immunohistochemical staining.
The antibody has been used to detect AR by various procedures, including
immunocytochemistry, immunohistochemistry and Western blot. By Western
blot, this product detects a ~110 kDa band representing AR, faint bands at
70 and 75 kDa are also seen in pre-immune sera. Immunohistochemical
studies of both frozen and formalin-fixed, paraffin-embedded (FFPE) sections
show intense nuclear staining consistent with nuclear localization of AR.
With FFPE sections, antigen retrieval methods using microwave fixation must
be utilized. New Zealand White Rabbits were immunized with the synthetic
peptide corresponding to the N-terminal 21 amino acids of rat and human AR.
An additional cysteine residue was added at the C-terminal end for
conjugation to KLH. Specific antibody against the antigenic peptide was epitope
purified by affinity chromatography.
Peptide sequence: M(1) E V Q L G L G R V Y P R P P S K T Y R G(21) C
Uses & Suggested Working Concentrations
western blot: 4ug/ml
Optimal titer must be determined by the end user for their particular
Packaging: 100 ug of purified rabbit antibody in 0.1ml (1.0mg/ml) PBS buffer with 1mg/mL BSA and 0.05% sodium azide. Keep in concentrated form until use. Store at 4-8 DEG C DO NOT FREEZE
General related Reference:
G. S. Prins, L. Birch, G. L. Greene. Androgen Receptor Localization in Different Cell Types of the Adult Rat Prostate. Endocrinology, 129(6): 3187-3199, 1991.
For In vitro Research Use Only-Not For Use in Diagnostics.
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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