rev: July 15, 1999

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ANTIBODIES  

                                                                  (anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


PRODUCT: HUMAN AMYLOID PRECURSOR PROTEIN FRAMESHIFT

                      MUTANT C-TERMINAL PEPTIDE ANTISERUM



Cat. #  RDI-AMPREFRabG   $312.00/0.25ml

 -A goat antiserum to a synthetic peptide that corresponds to amino acids 339-348 of the C-terminus of the Alzheimer disease amyloid precursor protein frameshift mutant (APPFM) is currently available. The amyloid precursor protein in a large membrane protein whose N-terminus projects into the extracellular space. In Alzheimer's disease, this protein is broken down and releases the 42 amino acid b-amyloid protein that is found in senile plaques and vessels.

-A frameshift mutant of the amyloid precursor protein has been found that results from a GA dinucleotide deletion which results in a frameshift mutation that gives rise to a truncated amyloid precursor protein with a unique C-terminus.

-The role of the amyloid precursor protein frameshift mutant in Alzheimer’s disease is currently unknown. These abnormal proteins may accumulate and lead to cellular distrubances and neuronal degeneration. This antiserum has been shown to be immunoreactive to the immunizing peptide by ELISA. The antiserum will immunolabel dystrophic neurites in senile plaques in formalin-fixed, paraffin-embedded sections from Alzheimer brain. It also shows immunoreactivity with neurons containing the APP frameshift mutnat This antibody should be useful to scientists and neuropathologists who study the role of amyloid in Alzheimer’s disease.

-Reference: van Leeuwen, F.W., et al. Frameshift Mutants of  -Amyloid Precursor Protein and Ubiquitin-B in Alzheimer’s and Down Patients. Science 279: 242-247, 1998. 

DATA SHEET: POLYCLONAL ANTISERUM

LOT NO:

ANTIBODY HOST: Goat

 -IMMUNOGEN: Synthetic peptide corresponding to amino acids 339-348 of the C-terminal region of the human Alzheimer’s Amyloid Precursor Protein Frameshift Mutant (APPFM)

sequence: H M NV Q N G K W D D D P S G TK TC I  (cat#RDI-AMYPREF-CPX  $469.00/1mg)
 

FORM: Whole antiserum

CONCENTRATION: N/A

TITER: Recommended dilutions based on neat serum:
-ELISA using immunizing peptide:  1:5,000
-IHC formalin-fixed, paraffin sections:  1:300
-Western Blots: N/D
-IHC paraformaldehyde-fixed, frozen sections: N/D

-SPECIFICITY: Specific for residues 339-348 on the mid-portion of human Amyloid Precursor Protein Frameshift Mutant Protein

STORAGE: 4 DEG   C short term; -20 DEG C long term

FOR RESEARCH AND MANUFACTURING USE ONLY

 Alzheimer disease brain showing immunohistochemical staining of Senile Plaque at 1:300 dilution in formalin-fixed, paraffin-embedded section of hippocampus.



 RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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