rev:  June 17, 2001

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.  


Product:                  Rabbit anti- Amyloid Beta N-terminal Antiserum

Cat No:                  RDI-AMYLBNabR   $375.00/0.25ml

Host:                      Rabbit

Clone:                     Polyclonal

Immunogen:             Synthetic Peptide: 1 DAEFRHDSGYEVHH 14

Form:                      Whole serum with 0.1% sodium azide

                              Azide free (order cat#RDI-AMYLBNabG-XP  $1250.00/1ml)

Concentration:           N/A

Supplied as:              0.25 ml vials

 Titer:                       Reported working dilution using neat serum:*

                                           -ELISA using immunizing peptide: approx 1:1,000

                                 -IHC using formalin-fixed, paraffin-embedded sections: approx 1:200

                                           -IHC using paraformaldehyde-fixed, frozen sections: N/D

                                           -  Western blots: N/D

 

Specificity:                Amino acid sequence 1-14 of human amyloid Beta protein.

Storage:                   Short term: Refrigerate at 4º C (1-2 months)

Long term: Freeze at -20º C. (recommend aliquot and store –20 DEG C.       Avoid frequent freeze thaw cycles)

 FOR RESEARCH AND MANUFACTURING USE ONLY

 * Various assay conditions require that the optimum working concentrations be determined by serial dilutions of this product.


Product: Human Amyloid Beta N-terminal Peptide Antiserum

 Cat No: RDI-AMYLBNabR

 Amyloid Beta (ABeta) is the peptide that accumulates in the brain in Alzheimer's disease and certain classes of cerebrovascular angiopathy. Alzheimer’s disease (AD) is characterized by the progressive formation in the brain of insoluble amyloid plaques and vascular deposits consisting of the 4-kD ABeta-peptide.  ABeta generation is initiated by proteolytic cleavage of the amyloid precursor protein (APP) at the N-terminal of ABeta by Beta-secretase.  The ABeta peptide is then released by proteolytic cleavage at its C-terminus by (-secretase.  The insoluble ABeta peptide forms aggregates in the vessels and senile plaques of AD brains.

 A rabbit antiserum to a synthetic peptide that corresponds to amino acids 1-14 of the Nterminal sequence of Abeta has been produced and is currently available.  This antiserum has been shown to be immunoreactive with the unconjugated immunizing peptide by ELISA.  This antiserum is capable of detecting ABeta by immunohistochemistry or protein immunoblots and will identify it from other CNS amyloids. Using formic acid treatment of formalin-fixed, paraffin-embedded sections, this antiserum will detect both cerebrovascular and core Abeta in senile plaques. It will also detect ABeta on immunoblots of formic acid extracts of brain tissue. The immunohistochemical detection of ABeta has been shown to be more sensitive than histochemical methods.  This antibody should be a valuable tool for scientists working to understand the role of ABeta in Alzheimer’s disease.

 This antiserum was produced using proprietary methodology whereby the peptide is attached to a carrier that elicits minimal immunoreactivity so that the antiserum has a higher degree of specificity for the peptide.  Since there is no overwhelming production of interfering antibodies to the carrier, this antiserum can routinely be used without further purification.  A control immunizing peptide (RDI-AMYLBN-CPX  $312.00/1mg) is available to be used in conjunction with this antibody to neutralize immunoreactivity.

 

Amyloid Beta N-terminal Peptide Antiserum

Cat No. RDI-AMYLBNabR

 

 Alzheimer disease brain showing   immunohistochemical staining of vessels containing amyloid Beta deposits at 1:200 dilution in formalin-fixed, paraffin-embedded,  formic-acid-treated section of hippocampus.


 

 Alzheimer disease brain showing immunohistochemical staining of amyloid cores in senile plaques containing amyloid Beta deposits at 1:200 dilution in formalin-fixed,  paraffin-embedded, formic-acid-treated section of hippocampus.

  

For Research Use Only

 

See other antibodies at:

http://www.researchd.com/absort.htm


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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