rev: October 11, 2004
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(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat# RDI-TYROHabmX $500.00
Presentation: 100ul of reconstituted supernantant with 15mM sodium azide
Immunogen: Recombinant protein, C-terminal portion of mouse sequence
Species Reactivity: Rat, Human, Mouse (others not tested)
Applications: Immunohistochemistry 1:20-1:40
Western blotting 1:25 1:50
Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
Storage: Store frozen. Aliquot as undiluted serum and immediately place at -20 º C. Serum may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6 months at -20 º C. Repeated freeze/ thaw cycles compromise the integrity of the antiserum.
Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrodes solution, 2) paraformaldehye-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4º C with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.
Note: Sodium azide (NaN3) interferes with peroxidase reactions and should not be used with peroxidase methodologies. If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azide-free buffer before performing the peroxidase reaction.
FOR In Vitro RESEARCH USE ONLY
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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