rev:       August 11, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Human Serum Albumin (monoclonal and polyclonals, matched pairs below)

see also matched pairs for other species


MONOCLONAL ANTI-Human Albumin

catalog# RDI-TRK4T24-1C8      (for clone 1C8)

              RDI-TRK4T24-15C7    (for clone 15C7) 

              RDI-TRK4T24-1A9      (for clone 1A9)

              RDI-TRK4T24-6B11    (for clone 6B11)

$375.00/1mg    Bulk quotes on request

packaging: 1mg of purified antibody in 0. ml ( mg/ml)PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at 4 DEG   C.

Purification: Protein A sepharose. Purity tested by electrophoresis.

Clones(isotype) 1C8 (mIgG1)

                         15C7 (mIgG2b)

                         1A9 (IgG2a)

                        6B11 (mIgG2a)

derived from hybridization of SP2/o myeloma cells with spleen cells of Balb/c mice immunized with human albumin.

Specificity: -specific for human albumin. No cross reactivity with other human proteins tested. Does not react with bovine serum albumin and egg white albumin.

Application: Suitable for ELISA techniques or radiolabelling, purification of human serum albumin.

                    western blot (clones 15C7, 1A9 and 6B11)

                  -clone 6B11 is probably the best since it also is the only of the 3 to see HSA from normal human serum after protein separation by SDS-PAGE without b-mercaptoethanol (non- reducing conditions). All three work under standard reducing contions.

                   Best Pairs for Elisa:

                  Coating      Detection

                   15C7            1A9

                  15C7             6B11

Safety: Although this antibody contains low amounts of sodium azide (0.1%), appropriate care must be taken when using this product. Avoid use of metal drains and flush with copious amounts of water. All material is for in vitro research use only-not for use in or on humans or animals-not for diagnostics. Not responsible for any patent infringements for the use or derivation of these products.

Note: Upon receipt, tighten cap and centrifuge for 1-2 minutes at 1-2KRPM to concentrate antibody in vial. If desired, add PBS with 0.1% NaN3 to bring concentration to 1mg/ml.see also polyclonal for human albumin

See also our other anti-human and animal immunoglobulin antibodies (IgA, IgD, IgE, IgG & IgM).


DATA SHEET:

Rabbit anti-Human Serum Albumin, Affinity Purified

Catalog#: RDI-HSAabr-AP $312.00/vial   

Also available:

Rabbit anti-Human Serum Albumin, HRP, Affinity Purified

HRP conjugated: cat#RDI-HSAabr-HRP-AP $344.00/1mg

Rabbit anti-Human Serum Albumin, BIOTIN, Affinity Purified

Biotin labeled cat#RDI-HSAabr-BT-AP $344.00/1mg


Size: 1 mg (lyophilized)

Antibody Concentration: see batch sheet


Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2

Stablizer: None

Preservative: 0.01% (w/v) Sodium Azide


Antigen: human serum albumin


Applications: suitable for immunoblotting (western or dot blot),ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.


Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher.


Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstituion.


For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


-see matched pair poylclonals for Elisa cat#RDI-E80129B  $438.00


Goat anti-human Albumin cat#RDI-A80229AB  $250.00/0.5mg affinity pure ,

absorbed against bovine,mouse and pig

Goat anti-human Albumin :HRP cat#RDI-A80229PB  $281.00/0.5mg affinity pure

absorbed against bovine,mouse and pig

Goat anti-human Albumin:FITC cat#RDI-A80229FB  $250.00/0.5mg affinity pure

absorbed against bovine,mouse and pig

Goat anti-human Albumin:Alk Phos cat#RDI-A80229APB  $250.00/.1mg affinity pure

absorbed against bovine,mouse and pig

Goat anti-human Albumin :HRP      cat#RDI-A80129PB  $281.00/1mg affinity pure  $250.00/mg 2+

Goat anti-human Albumin:FITC       cat#RDI-A80129FB  $250.00/1mg affinity pure  $188.00/mg 2+

Goat anti-human Albumin:Alk Phos  cat#RDI-A80129APB  $250.00/1mg  affinity pure  $188.00/mg 2+

- Elisa Reagent Packs (specific for each following analyte)available for Human IgA, IgD, IgE, IgG, IgM, albumin,

transferrin , myoglobulin, fetal hemoglobin, hemoglobin, kappa, kappa free, lambda, lambda free

Please inquire set, includes coating ab, HRP-conjugated

secondary ab and a reference serum standard

Starter pack: contains microwell plates, buffers and TMB for use with above matched pair antibodies cat#RDI-E101PK $344.00


matched pairs antibodies for albumin (includes a coating antibody, a matching HRP-conjugated detecting antibody, a standard and a sample protocol for making your own Elisa, approx 1000 wells)

cat#RDI-E110113B     Bovine Albumin   $438.00

cat#RDI-E70122B       Horse Albumin     $438.00  - DISCONTINUED

cat#RDI-E80129B       Human Albumin    $438.00

cat#RDI-E90134B       Mouse Albumin   $438.00

cat#RDI-E100110B     Pig albumin         $438.00

cat#RDI-E110125B     Rat Albumin        $500.00

Starter pack: contains microwell plates, buffers and TMB for use with above matched pair antibodies cat#RDI-E101PK $344.00


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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