rev:   October 14,2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ALPHA Fetoprotein

(see also anti-alpha fetoprotein receptor antibodies)


Monoclonal mouse anti-human a-fetoprotein

CAT#: RDI-TRK4F16-5H7,     or RDI-TRK4F16-4A3

            -other clones on special request only

PRICE: $375.00/mg or  $344.00/mg 2-9   $312.00/mg 10+

Significant Bulk Discounts-please inquire

Clone: 5H7, 4A3

 These clones have been derived from hybridization of SP2/0 myeloma cells with spleen cells of a Balb/c mice immunized with purified human afetoprotein.

Specificity: MoAb do not cross-react with human albumin.

Mouse isotype:  IgG2a for clone 4A3    

                          IgG1 for clone 5H7    

Application:

MoAb 4A3 and 5H7 can be used in two-site immunoassay for detection of human a-fetoprotein.In sandwich we recommend to use MoAb 4A3 for coating and MoAb 5H7 for labeling. BEST PAIR

MoAb 7H8 can be used only for affinity purification of human a-fetoprotein.

Purification: Purified by chromatography on protein A Sepharose.

Purity is tested by electrophoresis.

Presentation: Each vial contains 1mg antibodies in 0.1ml (10.5mg/ml) PBS, pH 7.4,containing 0.1% of sodium azide as preservative. Store at +4C. SEE VIAl LABELS

Note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. FOR IN VITRO RESEARCH USE ONLY

NOTE: Recommend centrifuging the unopened vial at 500-1000 RPMfor 2 minutes to concentrate antibody in bottom of vial. If desired, you can add 0.9ml PBS containing 0.1% NaN3 to bring volume to 1mg in 1ml.


FOR IN VITRO RESEARCH USE ONLY. NOT FOR USE IN OR ON HUMANS OR ANIMALS-NOT FOR DIAGNOSTICS. Not responsible for any Patent infringements with use or derivation of these products.


Human Alphafetoprotein

CAT#: RDI-TRK8F8   $562.00/0.5mg   $1000/1.0mg

(approx 2 weeks for delivery after order).

Source: Human fetal cord serum. Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 ,HIV-2 and HCV antibodies.

Purification: Immunoaffinity chromatography, biospecific chromatography

Presentation: Lyophilized from 0.1M PBS pH 7.2 with 2.5% sucrose and 0.1% NaN3. It is recommended that the product should be reconstituted in distilled water. Dilution should be into other buffers containing 2.5% sucrose and/or serum treated to remove any proteases (or with protease free BSA etc).

Analysis: Purity more 98% according to SDS-PAGE Does not contain albumin, antitrypsin or transferrin

Immunological activity confirmed by reaction with monoclonals which are specific to human alphafetoprotein.

Storage: At -20'C in lyophilized form. After reconstitution the solution should be aliquoted and stored at- 20'C. Avoid frequent freeze thaw cycles.

For In Vitro Research Use Only-Not for Use in or on human or animals-not for use in diagnostics


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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