rev: December 23, 2004
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(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Description: Produced from sera of rabbits immunised with the recombinant
highly conserved C-terminal part of the homeobox transcription factor Prox1.
Prox1 is expressed in CNS, eye, pancreas, liver and heart, and it is one
of the most specific and reliable markers for lymphatic endothelial cells.
Host species: Rabbit
Antigen: Recombinant human C-terminal part of Prox1 (homeodomain and prospero domain)
Purification: Protein A chromatography
Buffer: lyophilized from PBS, pH 7.4 w/o preservative
Formulation: lyophilized rabbit IgG
Reconstitution: The lyophilized IgG is stable at 4°C. for at least one month and for more than a year when kept at -20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six weeks at 2-4°C in the presence of a preservative. Reconstituted antibody can also be aliquoted and stored at -20°C to -70°C for at least 6 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Immunostaining: This antibody is a very valuable tool to detect and analyse lymphatic endothelial cells of different species. It was tested for fish, chicken, mouse, rat and human lymphatic endothelial cells by different staining techniques and has been used in several publications before. Use at 5-10 ug/ml for cell immunostaining and staining of unfixed, frozen tissue sections.
-It has been reported to work in fixed tissues (even when embedded in paraffin see Mouta et al, Cancer Res 2001) as well as in frozen sections of fixed mouse tissues (Padera et al, Science 2002).
Optimal dilutions should be determined by each laboratory for each application.
ELISA: The antibody can also be used for ELISA applications at 1-10 ug/ml IgG
Usage: For In Vitro research Use Only-not for use in or on humans or animals
1) licenced from Stanislav Tomarev, NIH, Washington.
Literature: T. Belecky et al., Invest Ophthalmol Vis Sci 38, 1293 (1997); E. Glasgow and S.I. Tomarev, Mech. Dev. 76, 175 (1998); M. Rodriguez-Niedenfuhr et al., Anat Embryol 204, 399 (2001); J. Wilting et al., FASEB J 16, 1271 (2002); J. Krishnan et al., Cancer Res. 63, 713 (2003). Cancer RES 61, 8079(2001), Padera et al, Science 296, 1883 (2002)
See additional info at: http://www.researchd.com/miscabs/prox1ab.pdf
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
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