rev:     December 28, 2004

HOME (index page)

Return (alphabetical antibody page)


  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Nephrin (see below 2 guinea pig polyclonals)
Pab to Nephrin (Human/Mouse)

Cat#RDI-PROGPN2   $470.00

Category                    Guinea pig polyclonal (GP-N2)

Purification / Form    Whole antiserum

Immunogen                 Synthetic peptide (intracellular domain, aa1243-1256)

Description/Specificity   The antibody reacts specifically with nephrin, first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE). Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.

Immunolocalization     The antibody stains positively podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas.

Antigen recognized in      Human, mouse

species (tested so far)

Reactivities on Cultured Cell Lines (tested so far) PCL (podocyte cell line), M-1 (cortical collecting duct cells)

Application

o Immunoblotting (Western blot)

o Immunofluorescence microscopy on frozen tissue

o Immunohistochemistry on paraffin sections, only when using the microwave method

Working Dilution 1:50 for immunohistochemistry

                             1:500 for immunoblotting

Incubation Time   1 h at room temperature or over night at 2-8°C

Storage At 2-8°C

Quantity 100 µL; contains 0.09% sodium azide

References

Kestila M, Lenkkeri U, Mannikko M, Lamerdin J, McCready P, Putaala H, Ruotsalainen V, Morita T, Nissinen M, Herva R, Kashtan CE, Peltonen L, Holmberg C, Olsen A, Tryggvason K: Positionally cloned gene for a novel glomerular protein--nephrin--is mutated in congenital nephrotic syndrome. Mol Cell 1, 575-582 (1998).

Ruotsalainen V, Ljungberg P, Wartiovaara J, Lenkkeri U, Kestila M, Jalanko H, Holmberg C, Tryggvason K: Nephrin is specifically located at the slit diaphragm of glomerular podocytes.Proc Natl Acad Sci USA 96, 7962-7967 (1999).

Holthofer H, Ahola H, Solin ML, Wang S, Palmen T, Luimula P, Miettinen A, Kerjaschki D. Nephrin localizes at the podocyte filtration slit area and is characteristically spliced in the human kidney. Am J Pathol 155, 1681-1687 (1999).

Putaala H, Soininen R, Kilpelainen P, Wartiovaara J, Tryggvason K: The murine nephrin gene is specifically expressed in kidney, brain and pancreas: inactivation of the gene leads to massive proteinuria and neonatal death. Hum Mol Genet 10, 1-8 (2001).

Huh W, Kim DJ, Kim MK, Kim YG, Oh HY, Ruotsalainen V, Tryggvason K: Expression of nephrin in acquired human glomerular disease. Nephrol Dial Transplant 17, 478-484 (2002).

Aaltonen P, Luimula P, Astrom E, Palmen T, Gronholm T, Palojoki E, Jaakkola I, Ahola H, Tikkanen I, Holthofer H: Changes in the expression of nephrin gene and protein in experimental diabetic nephropathy. Lab Invest 81, 1185-1190 (2001).

For Reseach Use Only


Pab to Nephrin  (Mouse)

Cat#RDI-PROGPN1    $470.00

Category                      Guinea pig polyclonal (GP-N1)

Purification / Form     Whole antiserum

Immunogen                  Synthetic peptide (extracellular fibronectin domain, aa974-987)

Description/Specificity   The antibody reacts specifically with nephrin, first described as a kidney podocyte marker protein (MW 135,000 calculated from aa sequence data; apparent Mr 185,000 after SDS-PAGE). Nephrin is a transmembrane cell adhesion molecule located at the slit diaphragm.

Immunolocalization The antibody stains positively podocytes of the kidney, radial glial cells of the brain, Sertoli cells of testis, and beta islet cells of the pancreas.

Antigen recognized in species     Mouse

(tested so far)

Reactivities on Cultured Cell      PCL (podocyte cell line), M-1 (cortical collecting duct cells)

Lines (tested so far)

Application

o Immunoblotting (Western blot)

o Immunohistochemistry of frozen tissue

o Immunocytochemistry (immunofluorescence)

Working Dilution 1:50 for immunohistochemistry

              1:500 for immunoblotting

Incubation Time 1 h at room temperature or over night at 2-8°C

Storage At 2-8°C

Quantity 100µL; contains 0.09% sodium azide

References

Kestila M, Lenkkeri U, Mannikko M, Lamerdin J, McCready P, Putaala H, Ruotsalainen V, Morita T, Nissinen M, Herva R, Kashtan CE, Peltonen L, Holmberg C, Olsen A, Tryggvason K: Positionally cloned gene for a novel glomerular protein--nephrin--is mutated in congenital nephrotic syndrome. Mol Cell. 4, 575-582 (1998).

Ruotsalainen V, Ljungberg P, Wartiovaara J, Lenkkeri U, Kestila M, Jalanko H, Holmberg C, Tryggvason K: Nephrin is specifically located at the slit diaphragm of glomerular podocytes. Proc Natl Acad Sci USA. 96, 7962-7967 (1999).

Holthofer H, Ahola H, Solin ML, Wang S, Palmen T, Luimula P, Miettinen A, Kerjaschki D: Nephrin localizes at the podocyte filtration slit area and is characteristically spliced in the human kidney. Am J Pathol 155, 1681-1687 (1999).

Putaala H, Soininen R, Kilpelainen P, Wartiovaara J, Tryggvason K: The murine nephrin gene is specifically expressed in kidney, brain and pancreas: inactivation of the gene leads to massive proteinuria and neonatal death. Hum Mol Genet 10, 1-8 (2001).

Huh W, Kim DJ, Kim MK, Kim YG, Oh HY, Ruotsalainen V, Tryggvason K: Expression of nephrin in acquired human glomerular disease. Nephrol Dial Transplant 17, 478-484 (2002).

Aaltonen P, Luimula P, Astrom E, Palmen T, Gronholm T, Palojoki E, Jaakkola I, Ahola H, Tikkanen I, Holthofer H: Changes in the expression of nephrin gene and protein in experimental diabetic nephropathy. Lab Invest 81, 1185-1190 (2001).

For Research Use Only also see cat#RDI-PROGPN2


need a secondary antibody?


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Return (alphabetical antibody page)

Ordering terms