rev:   March 6, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-P97 ATPase
Mab to p97 ATPase

cat#RDI-PRO65278   $375.00/vial

Clone                             58.13.3

Category                       Mouse monoclonal

Form/Purification         culture supernatant concentrate

Ig Class                         IgG1

Antigen                         15S Mg2+ ATPase complex purified from Xenopus laevis ovary

Description/Specificity  p97 ATPase is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p. The antibody reacts with the p97 subunit of 15S MG2+ ATPase.

Pos Control:                immunoblots with protein extracts from all vertebrate tissues and cells

Antigen Recognized in Species (tested so far) All verterbrate cells and tissues tested so far; eg. Xenopus laevis;rabbit, rat, human, DOES NOT Cross react with the corresponding protein in budding yeast (Cdc48p)

Suitable for · western blotting (not recommended for immunofluorescence and immunoprecipiation) approx 1:1000 dilution

Dilution Buffer             0.15M PBS with 0.1% BSA and 0.1% Na-Azide (ECL:5% milk powder in TBST)

Working Dilution          1:1000-1:2000

Storage                         At 2-8°C (undiluted)

Volume                        0.5 ml

References

                                 Cell 82:905-915 (1995)

                                 Cell 82:895-904 (1995)

                                 J Mol Biol 223:557-571 (1992)

                                 Embo J 9:1757-1767 (1990)

For Research Use Only


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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