rev: October 26, 1998

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Bombesin  (polyclonal)
Pab to Bombesin

Cat#RDI-PRO11182    $312.00

Category                  Rabbit polyclonal

Purification/Form    Rabbit serum

Immunogen              Synthetic bombesin conjugated to bovine thyroglobulin

Description/Specificity This polyclonal antibody can be used for the study of endocrine differentiation in tumors of the respiratory tract.

Recommended Positive Control    Gut (peripheral nerves), fetal lung

Antigen Recognized          Human (also reported mouse, rat and frog)

Application Immunohistochemistry  Suitable for frozen and paraffin section

Proteolytic treatment recommended (see below)

Working Dilution 1:50 for immunohistochemistry

Dilution Buffer PBS with 1% albumin and 0.1% Na-Azide

Incubation Time 30 min at room temperature or overnight at 2-8°C

Storage At 2-8°C (undiluted) or at -20°C (aliquots)

Volume 0.25 ml

Protocol  for the use of anti-Bombesin polyclonal antibody on paraffin-embedded tissue sections:

FIXATION:

Small tissue blocks are fixed in 4% buffered formaldehyde (pH 7.6) at 4°C. The fixation time should not exceed 12 h.

During the embedding process temperatures should not exceed 58°C.

STAINING PROCEDURE:

Deparaffinize and rehydrate sections.

If necessary, block endogeneous peroxidase activity (0.3% H2O2 in 50% methanol, 15 min). Rinse in PBS 3x.

Incubate for 5 min in 0.1% pronase in PBS at 37°C. Rinse in PBS 3x.

Apply properly diluted anti-Bombesin antibody (1:50) and incubate for 30 min at room temperature or overnight at 4°C. Rinse 3x.

Apply conjugated antibody. Rinse in PBS 3x.

Apply substrate.


need a secondary antibody?
RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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