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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Adipophilin  (Adipose differentiation-related protein)

(see below 3 forms , unpurified, purified and FITC conjugated)

see also new guinea pig polyclonal below  (mouse reactive)


Mab to Adipophilin

cat#RDI-PRO651102   $375.00/vial  

also available purified cat#RDI-PRO610102   $375.00/50ug  

FITC conjugated cat#RDI-PRO614102        $470.00/vial 0.25ml

Clone                                   AP125

Category                               Mouse monoclonal

Immunoglobulin Class          IgG1

Form/Purification                 Hybridoma culture supernatant

Antigen                                  synthetic peptide (aa 5-27 from N-terminus of human adipophilin)

Clinical Application/

Specificity New specific marker for liposarcoma. Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines, inducible by etomixir **

Polypeptide Reacting:        Adipophilin, Mr 48,100.13 (calculated from aa sequence data) apparent Mr 52,000 (after SDS-PAGE); pI 6.72

Immunolocalization:        Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver, adipocytes are negative. Specific detection of liposarcoma. Also positively stained are lipid storing CD68 positive macrophages

Antigen Recognizied:     Human, rat, dog (negative with bovine and mouse)

Reactivities on cultured Cell Lines: Caco, PLC, HaCat, SV80, RD 125, Huvec (human umbilical cord endothelial), RV, PC-12 (rat adrenal gland), MDCK; negative with glioma

Application                      o Immunoblotting  o Immunohistochemistry on frozen tissue, paraffin embedded after high t                                                  temp/microwave treatment

Working Dilution:             Ready to Use for immunohistochemistry  (unpurified)

                                         -approx 1:10 for purified (50ug in 1ml)

Storage                              At 2-8 DEC C

Volume                              5 ml (unpurified) or 50ug purified or 0.25ml (50ug FITC conjugated)

References

Hope, R.G. and McLauchlan J. Sequence motifs required fior lipd droplet association and protein stability are unique to the hepatitis C virus core protein. J General Virology 81, 1913-1925 (2000)

Heid, HW,et al, Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res 294:309-321 (1998)

Heid HW, Schnolzer M and TW Keenan. Adipocyte differentiation-related protein is secreted into milk as a constituent of milk lipid globule membrane. Biochem J 320:1025-1030 (1996)

Mrozinzki, J et al, Adipophilin and perilipin, two lipid droplet-associated proteins, in the pathohistological diagnosis of human soft tissue carcinomas. Abstract from AEK meeting 1999, German Cancer Research Center, J Cancer Res & Clin Oncol 125 suppl (1999)

Steiner S, Wahl D, Mangold BL, Robiron R, Raymackers J, Mehens L, Anderson NL, and Cordier A. Induction of the adipose differentiaion-related protein in the liver of rats. Biochem Biophys Res 218:777-782 (1996)

Hope, R.G. and McLauchlan J. Sequence motifs required fior lipd droplet association and protein stability are unique to the hepatitis C virus core protein. J General Virology 81, 1913-1925 (2000)

Hope RG et al, The domains required to direct core proteins of hepatitis C virus and GB Virus-B to lipid droplets share common features with plant oleosin proteins  J Biol Chem 277, 4261-4270 (2002)

Targett-Adams et al,  Live Cell analysis and targeting of hte lipid droplet binding protein ADRP, J Biol Chem in press (2003)

For In Vitro Research Use Only


Guinea Pig Pab to Adipophilin   (HUMAN/MOUSE/Canine/RAT/Bovine)

human homologue to mouse adipocyte differentiation-related protein (ADRP)

cat#RDI-PROGP40      $470.00

Category                           Guinea pig polyclonal (GP40, mN1)

Purification / Form          Whole antiserum

Immunogen                        Synthetic peptide (N-terminal aa 1-25 of murine adipophilin)

Description/Specificity    New specific marker for liposarcoma. Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir*.

Polypeptide reacting: Adipophilin, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72

Immunolocalization      Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Specific detection of liposarcoma.

Also positively stained are lipid-storing CD 68-positive macrophages.

Antigen recognized species            Human, mouse, rat, dog, bovine

(tested so far)

Reactivities on Cultured Cell           Caco, PLC, HaCat, SV80, RD 125, Huvec

Lines (tested so far)                          (human umbilical cord endothelia), RV, PC-12 (rat adrenal gland),

                                                         MDCK; negative with glioma

Application                                   · Immunoblotting (Western blot)

                                                    · Immunohistochemistry of frozen tissue, paraffin-embedded tissue

                                                       after microwave    treatment

                                                      · Cytological material

Working Dilution                        1:100 for immunohistochemistry  1:2000 for immunoblotting

Incubation Time 1 h at room temperature or over night at 2-8°C

Storage At 2-8°C

Quantity 100µL; contains 0.09% sodium azide

References

Heid HW, Moll R, Schwetlick I, Rackwitz HR and Keenan TW: Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res 294:309-321 (1998)

Heid HW, Schnölzer M and TW Keenan. Adipocyte differentiation-related protein is secreted into milk as a constituent of milk lipid globule membrane. Biochem J 320:1025-1030 (1996)

Mrozinzki J, SchwetlicK I, Heid HW, Winter-Simanowski S, Holzbach A, Hinze R, Franke WW and Moll R. Adipophilin and perilipin, two lipid droplet-associated proteins, in the pathohistological differential diagnosis of human soft tissue sarcomas. Abstract from AEK meeting 1999, German Cancer Research Center. J.Cancer Res.& Clin.Oncol. 125 suppl. (1999)

Steiner S, Wahl D, Mangold BL, Robiron R, Raymackers J, Mehens L, Anderson NL, and Cordier A. Induction of the adipocyte differentiation-related protein in the liver of rats. Biochem Biophys Res 218:777-782 (1996)

Hope R.G. and McLauchlan J. Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein. J. General Virology 81, 1913-1925 (2000)

-Buechler C et al, Adipophilin is a sensitive marker for lipid loading in human blood monocytes. Biochem Biophys Acta 1532, 97-104 (2001)

-Imamura M et al, ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts. AM J Physiol Endocrinol Metab 283, E775-E783 (2002)

-Targett-Adams P et al, Live clel analysis and targeting of the lipid droplet binding protein ADRP, J Biol Chem, Manuscript M211289200 (2003)

For Research Use Only

See also  anti-guinea pig secondary antibodies


RDI Division of Fitzgerald Industries Intl

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

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