rev:  March 15, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Rabbit anti-Beta-Glucuronidase

cat# RDI-BGLUCURabr      $438.00/1mg    

Presentation: 1mg in 0.5 mL (2mg/ml) PBS, pH 7.2 with 2 mM sodium azide.

Derivation: raised in rabbit against E. coli GUS

Purity: >95% IgG by SDS poly- acrylamide gel electrophoresis.

Storage: undiluted at 4ºC, protected from light, it is stable for at least three months. For longer storage, divide the solution into single-use aliquots and freeze at -20ºC. Frozen aliquots are stable for at least six months. AVOID REPEATED FREEZING AND THAWING.

Use: Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. We suggest using serial dilutions from about 1:500 to 1:10,000 to determine the optimal conditions for your experiments.

Bacckround: The Escherichia coli b-glucuronidase (GUS) gene is commonly employed as a reporter gene in plant transformation. In comparison with other reporter enzymes, GUS has several advantages: 1) There is very little endogenous activity in many higher plants including tobacco, potato, soybean, wheat, rice and Brassica; 2) The enzyme is very stable; and 3) GUS activity can be assayed at a wide pH range. 1-3 GUS catalyzes the cleavage of a variety of b-glucuronides, including many chromogenic and fluorogenic substrates, thus allowing histochemical, spectrophotometric and fluorometric measurements of GUS gene expression. 4 Alternatively, the GUS enzyme may be detected with anti b-glucuronidase in tissue sections or Western blots. 5,6 .

References

1. Proc Natl Acad Sci USA 83, 8447 (1986)

2. EMBO J 6, 3901 (1987)

3. Plant Mol Biol Rep 5, 387 (1987);

    Mol Gen Genet 215, 38 (1988)

4. GUS Protocols:Using the GUS Gene as a Reporter of Gene   Expression, S.R. Gallagher, Ed., Academic Press, Inc. (1992);

5. Mol Cell Biol 6, 811 (1986)

6. Proc Natl Acad Sci USA 86, 1529 (1989).

For In Vitro Research Use Only

copyright by owner


Mouse anti-Beta Glucuronidase

cat# RDI-BGLUCURabm-5    $565.00/vial

Presentation: 1ml supernatant solution

Clone: 105

Isotype: mIgG1

Specificity: reacts with a non-active center epitope on human placental and liver B-glucuronidase. Does not cross react with rat B-Glucuronidase

Use: Useful for staining human B-Glucuronidase in Elisa, Western blotting and FROZEN sections. The antibody apparently reacts with the native enzyme only.

Dilution: approx 1:10 for histochemisty

Storage: up to 2 months at 4 DEG C, 1 year at -20 DEG C. Recommend making working aliquots and store -20 DEG C. Avoid frequent freeze thaw cycles.

For In Vitro Research Use Only


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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