rev:     July 6, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


BCL-2 antibodies (monoclonal and polyclonal)  see new multi-species mono below


Bcl-2 ANTISERA


Rabbit anti-Rat/Mouse BCL-2 -

cat#RDI-RTBCL2abr   $406.00/vial 0.1ml neat sera with 0.1% NaN3

-specific for rat and to a lesser extent mouse BCL2

-suitable for immunoprecipitation (3-5ul/1M cells), western blot (1:188-1:2000), histochemistry (1:800-1:1500 dilution for paraffin sections-recommend Bouins fixative rather than formalin, in rat nervous and lymph system see ref#Oncogene 9:1799-1805 and J. Neuroscience 15:6364-6376)

-recommend rat thymus as positive control for western blot

-recommend rat thymus or brain stem for histochemistry control, staining is cytoplasmic

-made against immunogen consisting of a synthetic peptide of aa 68-86 ( R T S P L R P L V A T A G P A L S P V) from mouse BCL-2 but highly conserved between mouse and rat BCL-2


Rabbit anti-Mouse/RAT BCL-2

cat#RDI-MBCL2abr    $406.00/vial 0.1ml neat sera with 0.1% NaN3

-specific for mouse and to a lesser extent rat BCL2

-suitable for immunoprecipitation, western blot, histochemistry (parafin sections-in mouse nervous and lymph system see

ref#Oncogene 9:1799-1805 and J. Neuroscience 15:6364-6376)

-positive control-recommend M1 mouse myeoblast cell line

-made against immunogen consisting of a synthetic peptide of aa 68-86 ( R T S P L R P L V A T A G P A L S P V) from mouse BCL-2 but highly conserved netween mouse and rat BCL-2


Mouse anti-BCL-2 Monoclonal ab  (human/rat/mouse and chick) (Discontinued)

cat# RDI-BCL2Mabm        $438.00/vial 50ug (0.2ml)

       RDI-BCL2Mabm-1      $750.00/vial 150ug (0.6ml)

Background: Human hematopoietic malignancies are often characterized by gene rearrangements which deregulate gene expression. The Bcl-2 gene (B-cell   leukemia/lymphoma 2) was discovered by walking the   chromosomal breakpoints t(14;18). this gene consists of two exons and several trasncripts that encode two different protein products, BCL-2Alpha and BCL- 2beta. Both are approx 20-30kDa, and differ at their C-terminal region. BCL-2 suppresses programmed cell death and this property appears to be modulated by homodimerization or association with BAX, a cell death promoter.


isotype: mouse IgG1


package: 50ug or 150ug purified IgG1 at 0.25mg/ml in 50% glycerol, 20mM sodium phosphate pH 7.5, 150mM NaCl, 1.5mM NaN3, 1mg/ml BSA


antigen: a fragment corresponding to amino acids 49-179 of HUMAN bcl-2 protein


USe: Suitable for western blot on human, rat, mouse, and chicken using approx 1 ug/ml ab on total cell lysate of mouse macrophages (positive control). Major band at Mw 26kDa. -immunofluorescence


Storage: Store at -20 DEG C upon receipt. Avoid frequent freeze thaw cycles. Recommend aliquoting. Avoid frequent freeze thaw cycles.

Gen REF: Hanada, M et al. 1995 J. Biol. Chem 270:11962-11969

                Tsujimoto, Y et al, 1986 Proc. Natl Acad Sci USA 83:5214-5218


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

copyright by owner


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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