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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Monoclonal anti-HUMAN Apolipoprotein D

cat# RDI-APODabm   $438.00/vial

Presentation: 1 ml lyophilized supernatant with 15mM sodium azide added. Reconstitute with 1 ml distilled water, let set at room temp C for at least 30 minutes. Mix well.

Clone: 36C6

Isotype: mIgG1

Antigen: Prokaryotic recombinant protein corresponding to the mature full length apolipoprotein D molecule.

Hybridoma: mouse myeloma (P3-NS1-Ag4-1)

Specificity: recognizes Human apolipoprotein D

USES: -Paraffin sections (using citrate buffer high temperature release), not for frozen sections

            -typical dilutions:1:40-1:80, 60 minute incubation at 25 DEG C. ABC detection

           -western blot:1:500-1:1000 with enhanced luminescence detection

Stain: granular staining of apolipoprotein D containing lipids

Controls: testis

Storage: Store lyophilized material at 4 DEG C. Store reconstituted material at 4 DEG C for immediate use. For long term use, recommend aliquoting and store at -20 DEG C. Avoid frequent freeze thaw cycles.

Background:Apolipoprotein D is a glycoprotein involved in the human plasma lipid transport system. It is mainly associated with high density lipoprotein particles and forms disulphide heterodimers with other apolipoproteins. The function of apolipoprotein D in the metabolism of plasma lipoproteins is unclear but the obeservation that this protein forms complexes with lecithin:cholesterol acyltrasnferase has led to the suggestion that apolipoprotein D may be involved in cholersterol esterification and transport of substrates and products of the reaction. Apolipoprotein D is expressed in a range of normal tissues including axillary apocrine glands, adrenal cortex and corpus luteum. Peripheral nerves, pituitary, testis, cerebellum and renal tubes are also positive. Apoliporpotein D expression has been described in malignant prostrate gland but is absent in the normal glands. It is also highly expressed in cyst fluid from females with gross cystic disease of the breast and may be a feature in some breast carcinomas.

References:

-Hall R E et al, Expression of the androgen receptor and an androgen responsive protein apolipoprotein D, in human breast cancer, British Journal of Cancer 74:1175-1180 (1996)

-Aspinall J O et al, Differential expression of apolipoprotein D and prostate specific antigen in benign and malignant prostate tissues, The Journal of Urology 154:622-628 (1995)

-Diez-Ita I et al, Expression and prognostic significance of apolipoprotein D in breast cancer, American Journal of Pathology 144 (2):310-320 (1994)

-Weech P K et al, Apolipoprotein D and cross reacting human plasma apolipoproteins identified using monoclonal antibodies, The Journal of Biological Chemistry 261(17):7941-7951 (19986)

FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS

-copyright by owner


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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