rev:       May 26, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Neurofilament NF-M Monoclonal Antibody

Catalog Number:    RDI-ALS24507      $344.00/100ul

Clone: 3H11

Quantity: 100ul

Description: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Host Species: Mouse

Specificity: 3H11 was raised against a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli (1). The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species. Recognizes intact NF-M strongly and specifically on immunoblots in all mammals and birds tested to date and also binds NF-M in cells in tissue culture and in sectioned material.

Applications: For immunofluorescence, use 3H11 diluted 1:100. For western blots use 3H11 diluted 1:1000.

Format: Sterile cell culture fluid plus sodium azide. 3H11 is an IgG1 class antibody with a kappa light chain. Supplied at a concentration of 1mg/ml.

Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.

Reference: 1. Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 1991.

For research use only; not for use as a diagnostic.

For research use only; not for use as a diagnostic.


RDI Div of Fitzgerald Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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