rev:        April 13, 2005 (updated histo data)

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Anti-human Anti-PARK7 (DJ-1) Polyclonal Antibody

Catalog Number: RDI-ALS24369     $312.00/100ug

                            RDI-ALS24369X   $1875.00/1mg

Lot Number:

Quantity: 1 mg/ml in PBS containing 0.02% sodium azide.

Description: Four chromosomal loci (PARK2, PARK6, PARK7, and PARK9) associated with autosomal recessive, early onset parkinsonism are known. Positional cloning within the refined PARK7 critical region recently identified mutations in the DJ-1 gene in PARK7-linked families. Mutations in the PARK7/DJ-1 gene cause autosomal-recessive Parkinsons disease.

Host Species: Rabbit

Specificity: Reacts with residues 167-189 (AIVEALNGKEVAAQVKAPLVLKD) of human PARK7 (DJ-1). Also detects PARK7 (DJ-1) in mouse brain. 82% sequence identity to mouse and rat DJ-1.

Applications: Western blot. Recommended dilution for Western blot 1:500-1:1,000. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

-tech notes: PARK7 (DJ-1) Westerns with this antibody. The standard Western performance in  human HEK293 cells, rat PC12 cells, and in mouse brain lysates is fine, revealing a single band  migrating just above the 22kD marker. However, overexposure of blots does reveal cross-reactive bands with higher molecular weight, which do not interfere with the interpretation of  standard Westerns.

There is sometimes a lower migrating band, we observe one around 16kD, but only in the  insoluble fraction, both in wild-type and knockout mice. So this is a true non-specific band, but in a fraction that is not containing much DJ-1 anyway.

-immunohistochemistry performed with the antibody show that it works excellent and strongly stains reactive astrocytes in both human and mouse. In addition, there is a moderate staining of Pick bodies.

No labelling of Lewy bodies in Parkinson's disease is observed. Lewy bodies are the pathological hallmark of Parkinson's. The loss of  function mutation of PARK7 in the disease causes the lack of staining in Lewy bodies..

  -see sample stains  http://www.researchd.com/miscabs/als24369-ihc.htm

Format: Affinity purified IgG

Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.

Reference: Bandmann O. Neurology. 2004 Feb 10;62(3):357-8.
                  Hedrich K. et al. Neurology. 2004 Feb 10;62(3):389-94.
                  Bandopadhyay R et al. Brain. 2004 Feb;127(Pt 2):420-30.
                  Bonifati V. et al. Neurol Sci. 2003 Oct;24(3):159-60..

For In Vitro Research Use Only-not for use in or on humans or animals-not for use in diagnostics


RDI Division of Fitzgerald Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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