rev:      December 1, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


rabbit anti-human Alkaline Phosphatase  (human intestine)

cat#RDI-ALKPHOSHabr  $375.00/50mg

cat#RDI-ALKPHOSH-HRP $438.00/20mg HRP conjugated

cat#RDI-ALKPHOSH-BT   $438.00/10mg Biotin conjugated

-reacts with alkaline phosphatase from human intestine showing a 60kda band against this enzyme under reducing conditions of SDS-PAGE. Reactivity with ther sources unknown.


DATA SHEET: (ALK Phos calf intestine)

Rabbit anti-Alkaline Phosphatase   Catalog#: RDI-ALKPHOSabr   $312.00/50mg

Sheep anti-Alkaline Phosphatase                   RDI-ALKPHOSabS   $312.00/50mg

Goat anti-Alkaline Phosphatase                     RDI-ALKPHOSabG   $315.00/50mg

Also available HRP conjugated: cat# add -HRP $344.00/20mg

-custom FITC and PE conjugates available-please inquire


Size: 50 mg (lyophilized)


Antibody Concentration: see batch sheet

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2

Stabilizer: None

Preservative: 0.01% (w/v) Sodium Azide

Antigen: calf intestine alkaline phosphatase

Specificity: no cross is observed against the bacterial enzyme. Cross reactivity will occur with alkaline phosphatases from other mammals and tissues.


Applications: suitable for immunoblotting (western or dot blot),ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.


Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher.


Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstitution.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


MOUSE MONOCLONAL ANTIBODY TO: HUMAN ALKALINE PHOSPHATASE

PRODUCT CODE      RDI-CBL119     $375.00/1mg

CLONE                                    327

ISOTYPE                                 IgG1

IMMUNIZING ANTIGEN        Calf intestinal alkaline phosphatase

FUSION PARTNER                 NSO myeloma cell line

SOURCE                                  Tissue culture supernatant

PURIFICATION METHOD    Protein A affinity chromatography

SPECIFICITY                      The antibody reacts with alkaline phosphatase from a number of species and specific for the intestinal enzyme form.

APPLICATIONS                  *Used in an APAAP complex for immunohistochemistry

                                              *ELISA development as a detection antibody

LOT SPECIFIC DATA  RDI-CBL119 CONT'D

PRESENTATION The monoclonal is presented at a concentration of 1mg in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Bulk quotes on request

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


DATA SHEET: Rabbit anti-Alkaline Phosphatase (E.Coli)

Catalog#: RDI-ALKPHOSECabr   $312.00/vial

Also available HRP conjugated: cat# add -HRP  $344.00/20mg

Size: 50 mg (lyophilized)

Antibody Concentration: 50mg lyophilized

Buffer: 0.02 M Potassium Phosphate, 0.15 M

Sodium Chloride,pH 7.2

Stabilizer: None

Preservative: 0.01% (w/v) Sodium Azide

Antigen: E. Coli alkaline phosphatase

Specificity: reacts with alkaline phosphatase from E. Coli (cross reactivity may occur with other bacterial sources) No cross observed with mammalian enzyme.

Applications: suitable for immunoblotting (western or dot blot),ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.

Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher.

Storage Conditions: Store vial at 4'C prior to restoration Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquotcontents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstitution.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.


Note: None of these antibodies has been tested with other isoforms of alkaline phosphatase. Generally, if you pretreat your sample with levamisole, this will greatly reduce most non intestinal alkaline phosphatase activity.

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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