rev:  October 18, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


7 clones
Mouse anti-ACE (CD143) human

-clone CG2, mIgG

-cat#RDI-CD143-CG2    $688.00/200ug

-Mab CG2 can stain ACE in paraffin embedded tissue sections after microwave expression. The antibody doe snto react with the native enzyme and is therefroe not suitable for cryosections or functional studies. CD143 is restricted mainly to luminal endothelial cells of small muscular arteries and arterioles. On the other hand, practically all capillaries of normal human lung express CD143, while normal human kidney lacks any endothelial CD143 expression.

-ref: S. Danilov et al, J. Biol. Chem 269:26806

-Leucocyte Typing IV, 749, Garland Publishing, New York & London (1997)


mouse anti-Angiotensin Converting Enzyme


Catalog#: RDI-ACEabm

Cost: **** $500.00/100ug Bulk quotes on request

                   $438.00/vial 5 or more


LOT#

Packaging: 100ug purified in PBS pH 7.4 with 0.1% sodium azide added

Species: mouse IgMk

Source: ascites

Purification Method: Protein G affinity chromatography

Antigen: Purified ACE from rat lung

USE/Activity: reacts with human, bovine and mouse ACE and enthothelial cells and may be used in ELISA, immunohistochemistry and flow cytometry.

Storage: Store at 4 DEG C. -20 DEG C Long term

AVOID FREQUENT FREEZE THAW CYCLES.


For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the useor derivation of this product.


Also available: antibodies against mouse renin, rat angiotensinogen,rat/mouse angiotenisogen, rat kidney antigens, human kidney juxtaglomeluar cells, rat/mouse/human angiotenisin converting enzyme and other....please inquire!!!


MOUSE ANTI-ANGIOTENSIN-CONVERTING ENZYME (ACE) (CD143)

MONOCLONAL ANTIBODY   (Human/Mouse/Rat/Monkey/rabbit/bovine/canine)

CAT# RDI-ACEabm-2E2     $562.00

Presentation: 100ug in 0.1ml (1mg/ml)PBS, pH 7.4, 150 mM NaCl   containing 0.05% sodium azide.

CLONE: 2E2

ISOTYPE: IgG1

IMMUNOGEN: Angiotensin-converting enzyme (denatured) from human kidney

SPECIFICITY: Denatured Angiotensin-converting enzyme [ACE].

APPLICATIONS: -Immunohistochemistry on paraffin embedded tissue: 5 µg/mL.  

                              Western blotting: 5 µg/mL. Sensitivity: 100 mU/mL of human or rat ACE activity (1:20 dilution of                                     human ACE (Cat#RDI-ACE-AG) or rat ACE (cat#RDI-RTACE-AG) respectively, which correspond                                to 100 ng/lane)

                                Optimal working dilutions must be determined by the end user.

SPECIES REACTIVITIES: Human, monkey, rabbit, bovine, canine, feline, guinea pig, rat and mouse.

Storage: Store at -20 DEG C Long term AVOID FREQUENT FREEZE THAW CYCLES. At first thaw, tighten cap, centirufge 1-2minutes 1000rpm . Recommend making working aliquots with 0.1% BSA or other suitable carrier.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.


MOUSE ANTI-ANGIOTENSIN-CONVERTING ENZYME (ACE) (CD143)

MONOCLONAL ANTIBODY   (Human/Monkey/Rabbit/Bovine/Canine/Feline/GP)

CAT# RDI-ACEabm-3C5   $562.00

Presentation: 100ug in 0.1ml (1mg/ml)PBS, pH 7.4, 150 mM NaCl containing 0.05% sodium azide.

CLONE: 3C5

ISOTYPE: IgG1

IMMUNOGEN: Angiotensin-converting enzyme (denatured) from human kidney

SPECIFICITY: Denatured Angiotensin-converting enzyme [ACE].

APPLICATIONS: -Immunohistochemistry on paraffin embedded tissue: 5 µg/mL.

                       -Western blotting: 5 µg/mL. Sensitivity: 50 mU/mL of human ACE activity (1:50 dilution of purified human

                      ACE (Cat#RDI-ACE-AG) which correspond to 40 ng/lane)

                       Optimal working dilutions must be determined by the end user.

SPECIES REACTIVITIES: Denatured human, monkey, rabbit, bovine, canine, feline and guinea pig.

Storage: Store at -20 DEG C Long term AVOID FREQUENT FREEZE THAW CYCLES. At first thaw, tighten cap, centirufge 1-2minutes 1000rpm . Recommend making working aliquots with 0.1% BSA or other suitable carrier.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.


BATCH SHEET: mouse anti-Angiotensin Converting Enzyme   (human/Monkey/Rat/Hamster/cat)


Catalog#: RDI-ACENabm-9B9

Cost: **** $469.00/100ug Bulk quotes on request

Packaging: 100ug purified in 0.1ml (1mg/ml) in 10mM Borate buffer pH 8.0 and 150mM NaCl. (no preservative added

Species: mouse Ig1

Clone ref: 9B9

Antigen: Purified ACE from human lung

USE/Activity: reacts with human, monkey, rat, hamster and feline ACE and may be used as follows: in

     -immunoblotting: 1:100

     -Elisa:1:1000

     -immunohistochemistry:1:1000 on fresh frozen or 4% PFA fixed tissues (does not work on paraffin)

     -cytochemistry:1:100. Reacts with endothelial cell, macropgahes(activated) and THP1 cells (monocyte line)

     -immunoprecipitation:1K-1:10K

     -visualization of lung vessels

      -antibody mediated drug delivery into the lung

ref: Histochemistry (1987) 87:487-490

      Hypertension (1995) 25:219-226

      J. Clin. Invest (1995) 96:1823-1833

      PNAS USA (1996) 93:5213-5218

      J. Biol Chem (1994) 269:26806-26814

Storage: Store at -20 DEG C Long term AVOID FREQUENT FREEZE THAW CYCLES. If appropriate for your system, add preservative as 0.1% sodium azide.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.


BATCH SHEET: mouse anti-Angiotensin Converting Enzyme    (human/Monkey)

Catalog#: RDI-ACENabm-5F1

Cost: $469.00/100ug Bulk quotes on request

           

Packaging: 100ug purified in 0.1ml (1mg/ml) in 10mM Borate buffer pH 8.0 and 150mM NaCl. (no preservative added)


Species: mouse Ig1

Clone ref: 5F1

Antigen: Purified ACE from human lung

USE/Activity: reacts with N-terminal ACE of human and monkey, ACE and may be used as follows:
-immunoblotting: 1:100

-Elisa:1:1000

-immunohistochemistry:1:100 on fresh frozen or 4% PFA fixed tissues does not work on paraffin)

-cytochemistry:1:100. Reacts with endothelial cell, macrophages(activated) and THP1 cells (monocyte line)

-immunoprecipitation:1K

-strcuture-function of ACE

ref: Histochemistry (1987) 87:487-490

      Hypertension (1995) 25:219-226

      J. Clin. Invest (1995) 96:1823-18

      J. Biol Chem (1994) 269:26806-26814

Storage: Store at -20 DEG C Long term AVOID FREQUENT FREEZE THAW CYCLES. If appropriate for your system, add preservative as 0.1% sodium azide.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.


BATCH SHEET: mouse anti-Angiotensin Converting Enzyme   (human/Monkey/guinea pig/rabbit/porcine)

Catalog#: RDI-ACENabm-3A5

Cost: $469.00/100ug Bulk quotes on request


Packaging: 100ug purified in 0.1ml (1mg/ml) in 10mM Borate buffer pH 8.0 and 150mM NaCl. (no preservative added)


Species: mouse Ig1

Clone ref: 3A5

Antigen: Purified ACE from human lung

USE/Activity: reacts with N-terminal ACE of human, monkey, guinea pig, rabbit and porcine ACE and may be used as follows:
-Elisa:1:1000

-immunohistochemistry:1:100 on fresh frozen or 4% PFA fixed tissues (does not work on paraffin)
-cytochemistry:1:100. Reacts with endothelial cell, macrophages(activated) and THP1 cells (monocyte line)
-immunoprecipitation:1K

ref: Histochemistry (1987) 87:487-490

      Hypertension (1995) 25:219-226

      J. Clin. Invest (1995) 96:1823-1833

     J. Biol Chem (1994) 269:26806-26814

Storage: Store at -20 DEG C Long term AVOID FREQUENT FREEZE THAW CYCLES. If appropriate for your system, add preservative as 0.1% sodium azide.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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