rev:    May 19, 2005

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  ANTIBODIES  

                                                      (anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


14.3.3  (see 4 antibodies below)
Mouse anti-14-3-3 Sigma (human)

cat# RDI-1433Sabm    $438.00/100ug

cat# RDI-1433Sabm    $688.00/200ug

Presentation: Protein G purified antibody in 10mM PBS pH7.4 with 0.2% BSA and 15mM sodium azide (200ug/ml) -BSA/Azide free cat#RDI-1433Sabm-XP $750.00/200ug

clone ref: 1433S01

Isotype: mIgG1

immunogen: recombinant human 14-3-3 sigma protein

Use: -immunofluorescnece

         -immunoprecipitation (using Protein G approx 2ug/mg protein

         -Western blot 1ug/ml 2hrs RT with chemiluminescence detection

         -histochemistry: acetone fixed forzen sections

         -formalin fixed paraffin sections (requires high temp antigen release for 10-20minutes with citrate buffer pH6.0 followed by cooling for 20minutes at room temp)

Optimal dilutions must be determined with each use

Positive control: HT29 or HeLa cells, skin

Localization: cytoplasmic

Background: Recognizes a protein of 26-30kDa, identified as 14-3-3 sigma. This Mab is highly specific to14.3.3 sigma and shows no cross-reaction with other isoforms of 14.3.3.14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1, and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo. Expression of 14-3-3 sigma is higher in normal epithelial cells.

For In Vitro Research Use Only


Mouse anti-14-3-3 Gamma (human)

cat# RDI-1433Gabm     $438.00/100ug

cat# RDI-1433Gabm1  $688.00/200ug

Presentation: Protein G purified antibody in 10mM PBS pH7.4 with 0.2% BSA and 0.09% sodium azide (200ug/ml) -BSA/Azide free cat#RDI-1433Gabm-XP $750.00/200ug

clone ref: CG31

Isotype: mIgG1k

immunogen: recombinant human 14-3-3 gamma protein

Specificity: reacts only with 14.3.3 Gamma and shows no corss reaction with other isoforms of 14.3.3

Use: -immunoprecipitation (using Protein G approx 2ug/mg protein

        -Western blot 1-2ug/ml 2hrs RT with chemiluminescence detection (approx 26-33kda)

Species: humnan, others not tested

Positive  control: HeLa

Localization: cytoplasmic

Background: 14-3-3 proteins are a family of small,widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. Expression of 14-3-3 gamma is higher in cells from vascular smooth muscle, skeletal and heart muscle. In Alzheimer’s disease and Down syndrome level of 14-3-3 gamma in brain increases significantly.

References: 1. Fountoulakis M, Cairns N, Lubec G. (1999) J. Neural Transm Suppl. 57: 323-335.

                     2. Autieri MV, et al. (1996) Cell Growth Differ. 11:1453-1460.

For Research Use Only


Rabbit anti-14-3-3 Beta (human/Mouse/Rat)

cat# RDI-1433BabRX       $438.00/100ug

cat# RDI-1433BabRX1     $688.00/200ug

Presentation: affinity purified antibody in 10mM PBS pH7.4 with 0.2% BSA and 0.09% sodium azide (200ug/ml)

Isotype: rabbit IgG

immunogen: a synthetic peptide from N-terminus of human 14-3-3 beta

Use: -immunoprecipitation 2-5ug/mg protein

        -Western blot 1-2ug/ml 2hrs RT with chemiluminescence detection (approx 26-33kda)

        -formalin fixed paraffin sections-approx 1:50-1:100 with amplified detection.

         Requires high temp antigen release with 10mM citrate pH6.0 for 10-20 minutes followed by cooling at RT for 20 minutes

Species: human, mouse and rat others not tested

Positive control: HT20 cells, NIH/3T3 cell, prostate cancer tissue, liver tumour and brain

Localization: cytoplasmic

Background:

For Research Use Only


Mouse anti-14-3-3 PAN/Broad (human)

cat# RDI-1433Babm       $438.00/100ug

cat# RDI-1433Babm1    $688.00/200ug

Presentation: Protein G purified antibody in 10mM PBS pH7.4 with 0.2% BSA and 0.09% sodium azide (200ug/ml) -BSA/Azide free cat#RDI-1433Babm-XP $750.00/200ug

clone ref: CG15

Isotype: mIgG1k

immunogen: recombinant human 14-3-3 gamma protein

Specificity: broadly reactive with beta, gamma, sigma and zeta isoforms of 14.3.3

Use: -immunoprecipitation (using Protein G approx 2ug/mg protein

      -Western blot 1-2ug/ml 2hrs RT with chemiluminescence detection (approx 26-33kda)

Species: human, others not tested

Positive control: HeLa

Localization: cytoplasmic

Background: 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein.

References:

1. Laronga C, Yang HY, Neal C, Lee MH. J Biol. Chem 2000.

2. Baldin V. (2000) Prog cell cycle res. 4: 49-60

3. Skoulakis EM, Davis RL. (1998) Mol Neurobiol 3: 269-284.

4. Roberts MR. (2000) Curr Opin Plant Biol 5: 400-405.

For Research Use Only






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RDI Div of Fitzgerald Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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