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(for in vitro research use only)
RDI Division of Fitzgerald Industries Intl offers a wide line of antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!
cat# RDI-PR3X-AG 100ug $500.00
RDI-PR3X-AGX1 10 X 100ug $3125.00
RDI-PR3X-AG1 1 X 1mg $3250.00
RDI-PR3X-AG10 10mg $25000.00
DESCRIPTION. Highly purified by chromatography. Free of elastase and cathepsin
G. Prepared from 0.03 M Citric acid pH 3.5 + 1 M NaCl and <0.1% Triton®
X100 ,lyophilization after dialysis vs. 1mM Citric acid pH 3.5 + 0.005%
Triton(tm) X 100. The dried product contains approximately 10% PR3 protein
and 90% solids. Re-dissolve with H2O to 1 mg/ml.
SOURCE. Leucocytes of Purulent Human Sputum.
MW: SDS-PAGE indicates MW = 29,000.
Purity > 95% by native electrophoresis at pH 4.3 (4) yielding predominately 2 bands that migrate one-half the distance in comparison to Cathepsin G. Elastase, Lysozyme and Myeloperoxidase are also compared. A similar electrophoretic pattern was first described by Baggiolini et.al. (2) for the three proteases.
Storage: Store frozen at -20°. Re-dissolve with H2O to 1 mg/ml. After reconstitution,may be freeze-thawed up to 5 times (avoid frequent freeze thaw cycles, recommend aliquoting and store -20 DEG C).
Activity Test: 35 - 45 units per mg of protein. One unit will hydrolyze 1mMole of Boc-Ala-Ala-Nva-SBzl per minute at pH 7.5 at 25°C. The assay buffer-substrate (2 ml) is 0.1 M MOPS pH 7.5 containing 0.5 M NaCl, 10% DMSO, 1 mM substrate and 0.1 mM 5,5'-dithiobis-(2-nitrobenzoicacid). Use 0.2 to 0.5 ug of enzyme. Read increase in absorbance at 405 nm. The 1.0 mM extinction coefficient is 13.1 in a 10 mM cell.The kinetics of PR3 hydrolysis of thiolbenzyl substrates is described by Kam et. al. (1)
For Research or Further Manufacturing Use Only
RDI Division of Fitzgerald Industries Intl
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