rev:   September 16, 2004

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!


Human PR3 (Proteinase-3)

cat# RDI-PR3X-AG      100ug $500.00

RDI-PR3X-AGX1         10 X 100ug $3125.00

RDI-PR3X-AG1            1 X 1mg $3250.00

RDI-PR3X-AG10          10mg $25000.00

DESCRIPTION. Highly purified by chromatography. Free of elastase and cathepsin G. Prepared from 0.03 M Citric acid pH 3.5 + 1 M NaCl and <0.1% Triton® X100 ,lyophilization after dialysis vs. 1mM Citric acid pH 3.5 + 0.005% Triton(tm) X 100. The dried product contains approximately 10% PR3 protein and 90% solids. Re-dissolve with H2O to 1 mg/ml.

SOURCE. Leucocytes of Purulent Human Sputum.

MW: SDS-PAGE indicates MW = 29,000.

Purity > 95% by native electrophoresis at pH 4.3 (4) yielding predominately 2 bands that migrate one-half the distance in comparison to Cathepsin G. Elastase, Lysozyme and Myeloperoxidase are also compared. A similar electrophoretic pattern was first described by Baggiolini et.al. (2) for the three proteases.

Storage: Store frozen at -20°. Re-dissolve with H2O to 1 mg/ml. After reconstitution,may be freeze-thawed up to 5 times (avoid frequent freeze thaw cycles, recommend aliquoting and store -20 DEG C).

Activity Test: 35 - 45 units per mg of protein. One unit will hydrolyze 1mMole of Boc-Ala-Ala-Nva-SBzl per minute at pH 7.5 at 25°C. The assay buffer-substrate (2 ml) is 0.1 M MOPS pH 7.5 containing 0.5 M NaCl, 10% DMSO, 1 mM substrate and 0.1 mM 5,5'-dithiobis-(2-nitrobenzoicacid). Use 0.2 to 0.5 ug of enzyme. Read increase in absorbance at 405 nm. The 1.0 mM extinction coefficient is 13.1 in a 10 mM cell.The kinetics of PR3 hydrolysis of thiolbenzyl substrates is described by Kam et. al. (1)

For Research or Further Manufacturing Use Only


-see also antibodies toANCA

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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