rev:    November 10, 2004

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  ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!


Neuron-Specific Enolase (human)

CAT#: RDI-TRK8NS3    $938.00/100ug

                                          $1500.00/0.5mg

                                          $2500.00/1mg

Source: Postmortal human brain

Source: Postmortal human brain Blood sample from the tissue donor was tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies and syphilis

Purity: More than 95% (SDS-PAGE).

Purification: Purified by ion-exchange and gel-filtration chromatography according to Pahlman S. et al "Tumor Biology:, 1984, v.5, pp 127-139.

Activity: Specific activity-985 umol/min mg at 25 DEG C determined in 45mM imidazole, pH 6.8, 45mM Kcl, 1.33mM Mg2SO4, 1mM 2-PG. One unit is defined as the amount of NSE concentrated 1 mol of 2-phospho-D-glycerate pe rmin at 25 DEG C(J. Biochem 87, 1587-1594, 1980) Subunit composition is gamma-gamma.

Immunological Activity: Was tested in Elisa and immunoblotting with anti-NSE antibodies

Presentation: Solution in 0.1M Tris-Hcl, 5mM Mg2SO4, 0.25M KCL, pH 5.1 adjusted with sodium phosphate. no preservative added.

Store -20 DEG C. Avoid repeated freeze-thaw cycles.

Protein Concentration: see actual batch data (approx 0.85mg/ml according to spectrophotometer using E280nm

(1mg/ml)=0.895 0.120ml

For Research Use Only

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. W are not responsible for any patent infringements that might result with the use of or derivation of this product


Neuron Specific Enolase  (HUMan)

Code Number RDI-SCP147-3   $625.00/100ug   $1375.00/1mg   $4375.00/5mg

Presentation Supplied liquid frozen in 0.01M Sodium Phosphate buffer, pH 7.4, containing 0.15M NaCl and 0.005M Magnesium Sulfate.

Source Material Human brain tissue.

Composition

Specification > 96% Purity.

Testing Starting material is tested and found negative for HIV 1 and 2 antibodies, Hepatitis B surface antigen and HCV antibodies.

Analysis NSE level assessed by Roche Elecsys. Product shows enolase activity with a phospho-glycerate substrate.Protein determined by method of Lowry and Optical Density at 280nm. SDS-PAGE shows a single band corresponding to a molecular weight of approx. 45kD.

Storage Below -15ºC.

Comment n/a

Notes For In Vitro Research Use Only


see also human NSE Eia kit cat#RDI-42010CAN  $625.00/1 96 well kit  $500.00/kit 5+

-results within: 2hours 1 step procedure

- detection limit: <1ug/liter

-measuring range: 1-150ug/liter

-sample volume: 25ul

-hook effect: no hook up to 15,000 ug/lier

-stability: up to 12 months from date of production

-standard range: 6-165 ug/liter

-incubation temp: 20-28 DEG C

-recovery: 96-109%

detection: 620nm or 405nm

refernece value: <13ug/ml


PRODUCT DATA SHEET


Monoclonal mouse anti-neuron specific enolase


CAT#: RDI-TRK4N6-5G10, or   RDI-TRK4N6-5E2,or

           RDI-TRK4N6-5A4,   or   RDI-TRK4N6-1C1

PRICE: $375.00/mg or   $344.00/mg   2 or more (any combination) Bulk on request


Clone: 5G10, 5E2, 5A4, 1C1

These clones have been derived from hybridization of SP2/0 myeloma cells with spleen cells of a Balb/c mice immunized with purified neuron-specific enolase from human brain.


Specificity: Neuron-specific enolase (NSE), human

Reactivity: MoAb react with 45 kD band corresponding to NSE in immunoblotting of human brain extracts

Specific antibody titer in indirect ELISA is 1/1, 000.000 for all clones. MoAb 5G10, 5A4 and 5E2 may be used for immunohistochemistry on formalin-fixed and paraffin-embedded sections (recommend clone 5G10). Recommended dilution is 1:100=1:300. Optimal dilution must be determined by experimentation.


All antibodies work in ELISA.

The following pairs of our anti-NSE clones can be used to make an ELISA: 1C1 - 5A4, 1C1 - 5E2 and   1C1 - 5G10. According our data best results were   obtained with MAb 5G10 direct coating and MAb   1C1-HRP conjugate. Sensitivity approx 2-5ng/ml

(clone 1C1 can be purchased HRP conjugated, cat#RDI-TRK4N6-1CHRP  $688.00/1mg, $625.00/mg 2+)


Mouse isotype: Clones 5E2, 1C1, 5A4 - IgG2a

                        clone 5G10 - IgG2b


Application: Detection of NSE in ELISA and immunohistochemistry.



Presentation:Each vial contains 1 ml of antibodies with concentration 1 mg/ml, in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store 4-8 DEG C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.


FOR IN VITRO RESEARCH USE ONLY


Non-neuronal enolase from human brain (NNE)

Catalog #RDI-TRK8NS4

$938.00/100ug   $1500.00/500ug   $2500.00/1mg

Source: Isolated from frozen human brain: Blood sample from the tissue donor was tested and found negative for HBsAg, HCV, HIV-1 and 2 antibodies, syphilis.

Purity: > 95 % according to 10 % SDS-PAGE

Purification: Purified by ion-exchange chromatography, gel-filtration and ammonium sulphate fractionation

Activity: Determined in 45 mM imidasole pH 6.8, 45 mM KCl, 1.33 mM Mg2SO4, 1 mM 2-PG.

Immunoreactivity: Does not react with anti-NSE in ELISA and WB

Presentation : Solution in 10 mM TRIS/HCL, 5 mM MgSO4, pH 7.5

Protein concentration : Determined by BCA method ("Pierce" USA)

Storage: - 20 °C

Application: This product is for in vitro research use or further manufacture.Not for use in or on humans or animals-not for use in diagnsotics


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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