rev:      April 4, 2005

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  HUMAN ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!


Myeloperoxidase   (from human sputum)

cat# RDI-MYELOP-AG        $375.00/0.5mg

       RDI-MYELOP-AGX     $562.00/1mg

       RDI-MYELOP-AGX1 $625.00 10 X 100ug

        Bulk quotes on request

Presentation: A lyophilized, salt-free, green soluble powder.

SOURCE. Leucocytes of Purulent Human Sputum

SPECIFICATIONS Highly purified by ion exchange and gel diffusion chromatography.Purity greater than 98% by PAGE and gel diffusion Store at 5° C. Stable 1 year. Absorbance ration 430nm/280 nm is 0.70 to 0.75 on 0.1 mg/ml in 0.05 M phosphate pH 7 buffer.

MW ca. 59,000 in SDS-PAGE indicating two protein subunits.MW ca. 120,000 on S-200 chromatography.

Units: 28-33 units per mg of protein.1 unit will decompose 1 µmole of H2O2 per minute at pH 7.0 and 25°C using 4-amino-antipyrine as hydrogen donor. The assay procedure is given in Methods.

For In Vitro Research or Further Manufacturing Use Only


Natural human Myeloperoxidase (leukocytes)

-cat#RDI-TRK8M80  $438.00/100ug  $750.00/0.5mg   $1125.00/1mg

Bulk on request lyophilized

-source: human donor leukocytes. Blood sample from donor is as tested and found negative for HBsAg, HV-1 and HIV-2 antibodies, HCV, syphilis.

.-purity >95% by SDS-PAGE, immunological reactivity conirmed with monoclonal antibody specific for MPO

-suitable for use as coating antigen in an anti-MPO autoantibody immunoassay

-Anti-neutrophil cytoplasm antibodies (ANCA) are serological markers for certain systemic vasculitides. These autoAbs have been subclassified into cytoplasmic (cANCA) and perinuclear (pANCA) specificities according to the indirect immunofluorescent staining pattern of ethanol-foxed neutrophils.

The major pANCA target was subsequently shown to comprise a myeloperoxidase (MPO), an enzyme contained inalpha or primary azurophilic granules. The MPO protein (MW ca 140Kda is a covalently linked complex of two alpha chains (MW ca 60kDa) and two beta chain (MW ca 14kDa). The purified MPO antigen can be used for the detection of anti-MPO autoantibodies in immunoassays. The anti-MPO autoantibodies are associated with the wide range of systemic vasculitides, eg. periarteritis nodasa, microscopic polyarteritis, pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-MPO autoantibodies are also reported in rheumatoid arthritis.

For In Vitro Research or Further Manufacturing use only


Human Myeloperoxidase (MPO) >96%

Code No. RDI-SCP257-3   $500.00/100ug   $1250.00/1mg   $1125/mg 2-9

Source Material Human neutrophils.

Disease State Testing Starting material donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, Hepatitis C antibodies and Syphilis.

Product Presentation Single homogenous batch, supplied liquid frozen in 50mM sodium acetate buffer, pH 6.0, containing 0.1M sodium chloride.

Specifications >96% purity.

Analysis Native 4-20% - Polyacrylamide Gel Electrophoresis shows one band corresponding to MPO molecular weight.Total protein concentration as determined by Coomassie.

Manufacturing Quality System Mfg under a quality management system, which complies with the requirements of ISO 9001:2000, certificate (data on contract)

Storage 2-8°C.

For In Vitro Research or further manufacturing use only


Human Myeloperoxidase (MPO) >90%

Code No. RDI-SCP257-5    $625.00/1mg    $400.00/mg 3-9   $2750/10mg

Source Material Human neutrophils.

Disease State Testing Starting material donor tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, Hepatitis C antibodies and Syphilis.

Product Presentation Single homogenous batch, supplied liquid frozen in 50mM sodium acetate buffer, pH 6.0, containing 0.1M sodium chloride.

Specifications >90% purity.

Analysis Native 4-20% - Polyacrylamide Gel Electrophoresis shows one band corresponding to MPO molecular weight.Total protein concentration as determined by Coomassie.

Manufacturing Quality System Mfg under a quality management system, which complies with the requirements of ISO 9001:2000, certificate (data on contract)

Storage 2-8°C.

For In Vitro Research or further manufacturing use only


RDI Division of Fitzgerald Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

sales@researchd.com

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