rev: December 10, 2001
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Cytokines, Growth Factors & Chemokines:
(Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
see also new specialty VEGFR1 receptors
see also new Elisa kits for sVEGFR1 (total), sVEFFR1(free), VEGF-A
Cat#RDI-300-044 $312.00/5ug $250.00/vial
Description: A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His -tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa.
Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994). Different isolates of Orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appears to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999; Wise et al., 1999). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E can not bind to VEGF receptor-1 (Flt -1). Furthermore VEGF-E can also not bind to VEGF receptor-3 (FLT -4). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor -2/KDR.
Molecular Weight: ~35 kDa
Purity: > 90%, by SDS-PAGE and visualised by silver stain
Endotoxin level: < 0.1 ng per ug of VEGF-E
Biological Activity: The ED50 for stimulation of 3 H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF-E has been determined to be in the range of 5 - 20 ng/ml.
Reconstitution: The lyophilised VEGF-E is soluble in water and most aqueous buffers. The lyophilised VEGF-E should be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.
Stability: Lyophilised samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF-E should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
Usage: VEGF-E is offered for research use. Not for drug use. Not for human use!
Catalogue number: RDI-300-04
Range: 1-30 ng/ml
Literature: [Dehio et al., 1999 EMBO J. 18:363-374; Lyttle et al., 1994 J. Virol 68:84-92; Wise et al., 1999 Proc. Natl. Acad. Sci USA 96:3071-3076]
For Research Use Only
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RDI Division of Fitzgerald Industries Intl
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phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266