rev:      May 23, 2005

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Cytokines, Growth Factors & Chemokines:ANTIBODIES  

(anti-Human and others as indicated)

RDI Division of Fitzgerald Industries Intl  offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


TNF-A  (TUMOR NECROSIS FACTOR-ALPHA)

see antibodies against human/mouse/rat / & rabbit ,  below! see also human TNF alpha Elisa kit

 -For Elispot/bulk Elisa also see the Pelipairs(tm)


PRODUCT DATA SHEET

Monoclonal mouse anti-human a tumor necrosis factor

CAT#: RDI-TRK4T10-2C8,   or     RDI-TRK4T10-1D9, or

            RDI-TRK4T10-F6C5

PRICE: $375.00/mg  ,  $344.00/mg  2 or more (any combination) $312.00/mg 10+ (1 clone)

Clone: 2C8, 1D9,F6C5

These clones have been derived from hybridization of X63,Ag8.653 myeloma cells with spleen cells of a Balb/c mice immunized with human recombinant tumor necrosis factor of a type.

Reactivity: MoAb F6C5 directed to different TNF-a epitope than clones 2C8 and 1D9. MoAb 2C8 and 1D9 are effective in inhibition of the biological activity of recombinant and natural TNF-a.

Mouse isotype: All clones IgG1, kappa

Application: Inhibition of biological activity of TNF-a in different biological fluids. Detection of TNF-a in two site immunoassay. We recommend to use MoAb 2C8 for coating and F6C5 for labeling.

Detection of  TNF-a in immunohistochemistry.All clones work in immunohistochemistry.

Presentation:Each vial contains 1 ml of antibodies with concentration 1 mg/ml, in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at -20C. Avoid repeated freeze-thaw cycles.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

FOR IN VITRO RESEARCH USE ONLY


Goat anti-human TNF-A (antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-TNFAabGP   $250.00/50ug

cat#RDI-TNFAabGP1  $375.00/100ug

Goat anti-human TNF-A:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-TNFAabGPB  $250.00/25ug

cat#RDI-TNFAabGPB1  $375.00/50ug

Rabbit anti-murine TNF-A (antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-mTNFAabrP    $250.00/50ug

cat#RDI-mTMFAabrP1   $375.00/100ug

Goat anti-murine TNF-A:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-mTNFAabGPB    $250.00/25ug

cat#RDI-mTNFAabGPB1   $375.00/50ug

Rabbit anti-RAT TNF-A (antigen aff pure, lyophilized, no carrier/no preservative)

cat#RDI-RTTNFAabrP     $250.00/50ug

cat#RDI-RTTMFAabrP1   $375.00/100ug

Rabbit anti-RAT TNF-A:Biotin conjugated (antigen aff pure, conjugated to Biotin, lyophilized, carrier free)

cat#RDI-RTTNFAabrPB    $250.00/25ug

cat#RDI-RTTNFAabrPB1   $375.00/50ug


Mouse anti-human TNF-Alpha

cat# RDI-TNFAabm-EL1 $375.00  

Presentation: 500ug Lyophilized from PBS.

Isotype: Mouse IgG1

Derivation: Monoclonal antibodies were produced in BALB/c x C57BL/6 F1 mice using recombinant human TNF-alpha is he immunizing antigen. IgG1/K antibody was purified from ammonium sulfate precipitation followed by ion exchange.

Stability: The lyophilized antibody, though stable at room temperature, is best stored desiccated at -20 DEG C. Reconstituted anti-human TNF-alpha is stable at 40C for one month or can be stored in working aliquots at -20 DEG C.

Reconstitution: When reconstituted in sterile water to a concentration of 1.0 mg/ml the antibody is stable for at least six weeks at 2-40C.

Use: Direct ELISA (coating antibody)

For research use only. Not for use in diagnostic or therapeutic procedures.


Rabbit anti-HUMAN TNF-ALPHA

Catalog#: RDI-TNFAabr         $500.00/vial

LOT# see sheet with shipment

Package Size: 1.0 milligram purified via Protein A or ammonium sulphate precipitation in 1ml PBS buffer pH 7.4 (preservative and carrier free)

Antigen: recombinant human TNF-A

Species: Rabbit IgG

Activity: Reacts with human TNF-A (natural and recombinant forms). There is less than a 1% cross with human TNF-Beta (via ELISA) . No cross with other human cytokines.


Application

-Neutralization: 20ug/ml of lot#    will neutralize approx 100% of the activity of 0.5ng/ml and 50% of the activity of 4 ng/ml of recombinant human TNF-alpha as measured in the L929 bioassay.

-Western blotting: approx 10ug/ml per 100ng   of cytokine

-RIA/ ELISA

-immunoprecipitation


Endotoxin: approx 0.1EU/ml by LAL


Storage: Store at -20 Deg C. Avoid repeated freeze thaw cycles. For dilution, we recommend sterile PBS or media. A carrier protein (BSA) or serum should be added to diluted antibody to maintain the protein concentration at 1mg/ml or greater. This reduces antibody loss by blocking non specific binding to container surfaces.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.


Also available: 3 monoclonals against human TNF-alpha (for ELISA, immunohistochemistry & neutralization) see RDI-TRK4T10-



also available:

recombinant human TNF-A   cat#RDI-301         $235.00/50ug

recombinant mouse TNF-A   cat#RDI-3101A    $235.00/20ug

recombinant rat TNF-A         cat#RDI-4014      $231.00/20ug


Rabbit anti-Mouse/Rat TNF-Alpha


Catalog#: RDI-RTTNFAabr    $500.00/vial   


LOT#

Package Size: 0.5mg purified rabbit immunoglobulin with 125uMol trehalose (stabilizer)


Antigen: recombinant mouse TNF alpha form E. Coli


Biological activity: 8 X 105 neutralizng units/mg protein (mouse) 4 X 105 " " " " for rat


Species: rabbit


Specifiicty: neutralizes both natural and recombinant TNF- Alpha. This antibody shows a high degree of cross neutralizing activity with RAT TNF-A.

Application

-Neutralization: tested in a cytotoxic bioassay assuming 1 unit of TNF-alpha bioactivity equals 50 picograms TNF-alpha .

-ELISA

-Immunohistochemistry on frozen sections (recommend dialyzing before use to remove trehalose)


Dilutions: Any dilutions should be made in buffered solutions containing carrier protein such as PBS with 0.1 to 1.0% BSA.


Storage: Store at - 20 DEG C. Avoid repeated freeze thaw cycles.


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.


DATA SHEET: Rat anti-mouse TNF alpha (capture ab)

Catalog#: RDI-mTNFabrt-26   $500.00/vial

Package Size: 0.5mg in 1ml 10mM PBS pH7.2 with 150mM NaCl and 0.09% sodium azide)

Clone: G281-2626

Immunogen: insect cell expressed rec mouse TNF-A

Ig Isotype: rat IgG1

Purification: Purified from TCS by Protein G

Reactivity: Can be used as a coating antibody in a cytokine Elisa in conjunction with the biotin conjugated clone MP6-XT3 (cat#RDI-MTNFabD-BT, $562.00/0.5mg) Reacts with mouse TNF-alpha. Cross with mouse TNF-beta has not been tested. Cross with Rat TNF-A has been reported

Use: For best results, use a 0.1M sodium phosphate pH6.0 binding/coating buffer with this product Titer from 5-10ug/ml. In paired Elisa with clone MP6-XT3 no cross observed with other tested cytokines.

Storage: Store at 4 Deg C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

It is the responsibility of the user to comply with all local/state nd Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of orderivation of this product.


DATA SHEET: Rat anti-mouse TNF alpha-Biotin Labeled

Catalog#: RDI-mTNFabd-BT    Price: $562.00/500ug package

Package Size: 0.5mg in 1ml (0.5mg/ml)in 10mM PBS pH7.2 with 150mM NaCl and 0,09% sodium azide.

Clone: MP6-XT3

Ig Isotype: rat IgG1

Purification: Purified from TCS by affinity chromatography, coupled with NHS-Biotin, then dialyzed to remove remaining free biotin.

Reactivity: Can be used as a detecting antibody in a cytokine Elisa in conjunction with the purified capture (coating) antibody clone G281-2626, cat#RDI-MTNFAabrt-26 $500.00/0.5mg). Reacts with mouse TNF-alpha. Cross with mouse TNF-beta has not been tested. Cross with rat TNF-A has been reported .

In Elisa: titrate from 0.5-2ug/ml diluted in BSA or Biotin free casein buffer. Use 100ul per well.

Storage: Store at 4 Deg C. DO NOT FREEZE

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:    Rat anti-Mouse/Rat TNF-Alpha (ELISA)

Catalog#: RDI-MTNFAabrt   $500.00/vial

Package Size: 2.0 mg protein G purified antibody in 1.0ml 50mM Tris-HCL buffer, pH 8.0 with 150 mM NaCl & 0.1% NaN3.

Antigen: insect cell expressed mouse TNF-A

Clone ref: G281-2626

Species: rat IgG1

Specificity: recognizes mouse and RAT TNF-alpha

Application -ELISA as the coating/capture antibody (approx 1-10ug/ml). Must optimize for each application

-For mouse assay, pair with biotin labeled clone MP6-XT3.

( Rat anti-mouse TNF alpha-Biotin Labeled, Catalog#: RDI-mTNFabd-BT $625.00/500ug package)

  -incrases in sensitivity may be possible with use of our POLY-HRP40-Streptavidin 1:5-1:10K

-neutralization of mouse and rat TNF-A

-western blot: 1-5ug/ml under reducing conditions (<100ng/lane with mouse TNF-A).

Specificity: No cross found in Elisa pair tests with any murine, rat or human cytokine tested. Not tested against TNF-B for mouse or rat.

Storage: Store at 4 DEG C. DO NOT FREEZE.

Precautions: For In vitro research Use Only. Not   for use in or on humans or animals or for diagnostics.


Mouse anti-RABBIT TNF-Alpha:Biotin Labeled  


Catalog#: RDI-RBTNFAabm-BT     $562.00/vial   


LOT#

Package Size: 0.5mg Biotin conjugated antibody in buffered solution with 0.09% NaN3.


Antigen: E. Coli expressed rabbit TNF-A


Species: mouse

Specificity: recognizes rabbit TNF-alpha

Application -ELISA as the detecting antibody (approx 1- 8ug/ml). Must optimize for each application (use with non biotinylated ab approx standard curve from 10,000 pg/ml to 80pg/ml cat#RDI-RBTNFAabg as the coating antibody. $500.00/vial of 0.5mg


Storage: Store at 4 DEG C. DO NOT FREEZE.


Precautions: For In vitro research Use Only. Not   for use in or on humans or animals or   for diagnostics.


Goat anti-RABBIT TNF-Alpha  (for coating use)

Catalog#: RDI-RBTNFAabg    $562.00/vial


LOT#


Package Size: 1.0mg protein G purified antibody in 1ml 50mM Tris-HCL buffer, pH 8.0 with 150 mM NaCl

& 0.1% NaN3.


Antigen: E. Coli expressed rabbit TNF-A


Species: goat


Specificity: recognizes rabbit TNF-alpha


Application -ELISA as the coating/capture antibody (approx 4-10ug/ml). Must optimize for each application

(use with biotinylated ab approx standard curve from 10,000 pg/ml to 80pg/ml cat#RDI-RBTNFAabg-BT (Discontinued) as the coating antibody. $562.00/vial of 1.0mg


Storage: Store at 4 DEG C. DO NOT FREEZE.


Precautions: For In vitro research Use Only. Not   for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this products.


also available: rabbit TNF-A conditioned medium cat#RDI-RBTNFA   $312.00/80 nanograms in 1.5ml

Poly-HRP80-Streptavidin, Poly-HRPDIL & TMB-S

(excellent reagents for making an ultra-sensitive Elisa assay).


rev:Nov 1, 1995

Rabbit Tumor Necrosis Factor Alpha Conditioned Medium

Catalog#: RDI-RBTNFA   inquire
For In Vitro Research Use Only-Not For use in Diagnostics


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

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RDI Div of Fitzgerald Industries Intl

(Research Diagnostics Inc)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

email: sales@researchd.com

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