rev: December 23, 2003
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Cytokines, Growth Factors & Chemokines:ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of recombinant growth factors, cytokines and chemokines and antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
-rat IgG2a, clone ref#A75-2.1
-reacts with mouse, human and pig TGF-B1 (others not tested)
-immunogen: rec murine TGF-B1
-use: western blot: 1-5ug/ml to detect approx 100ng/lane human TGF-B1 with chemiluminescence detection .
-ELISA: Use as the coating antibody with cat#RDI-TGFB1MS-3BT , rat anti-TGF-B1:Biotin conjugated detecting antibody $625.00/0.5mg, clone A75-3.1, rat IgG2a). Use cat#RDI-102r human TGF-B1 as standard $212.00/5ug Note: In Elisa TGF-B1 must be pretreated by acidification. For serum samples, first dilute serum in PBS 1:5 then add 1N HCL 1:25 to adjust to pH3 (ie 4ul 1N HCL to 100ul diluted serum). Cell supernatants can be treated undiluted with 1N HCL 1L25 to adjust to pH3 (ie 4ul 1N HCL to 100ul superntant). Incubate acidified samples for 60 minutes at 4 DEG C (alternatively, samples can betreated at room temp for 15 minutes). After incubation neutralize samples by treating each sample with 1N NaOH 1:25 and test immediately or store at -80 DEG C.
see also POLY-HRP40-SA and RDI-TMB-S for ELISA use
-0.5mg Protein G purified mouse monoclonal, lyophilized, no carrier/no preservative
-suitable for Elisa or WB (1ug/ml)
For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.
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RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266