rev: January 23, 2002

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


Recombinant Human sVEGFR-3/FLT-4 )/Fc Chimera  (see also sVEGFR-3)
Recombinant Human sVEGFR-3/Fc Chimera

Description: Recombinant human soluble Vascular Endothelial Growth Factor Receptor-3 (sVEGFR-3) was fused with the Fc part of human IgG1. The recombinant mature sVEGFR-3/Fc is a disulfide-linked homodimeric protein. The sVEGFR-3/Fc monomers have a mass of approximately 130 kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Glu774).

All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin-like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The VEGFR-3/FLT-4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751 aa residue extracellular domain, a 22 aa transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4. The FLT -4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial at latter stages of development. It is important for lymphangiogenesis.

Source:                             Insect cells

Molecular Weight:          ~260 kDa

Subunit:                           glycosylated dimer

Purity:                             > 90%, by SDS-PAGE and visualised by silver stain

Endotoxin level:             < 0.1 ng per mg of soluble sVEGFR-3/Fc

Stabilizer:                       none

Buffer:                            none

Formulation:                   lyophilized

Specific Activity: Measured by its ability to bind recombinant rat VEGF-C in a functional solid phase binding assay. Immobilised recombinant human sVEGFR-3/Fc at 5 µg/ml can bind recombinant rat VEGF-C in a linear range of 8-500 ng/ml.

Reconstitution: The lyophilised sVEGFR-3/Fc is soluble in water and most aqueous buffers. The lyophilized sVEGFR-3/Fc should be reconstituted in PBS or medium to a concentration not lower than 100 µg/ml.

Stability: Lyophilised samples are stable for greater than six months at -20°C to -70°C. Reconstituted sVEGFR-3/Fc should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!

Usage: sVEGFR-3/Fc is offered for research use. Not for drug use. Not for human use.

Catalogue number: RDI-SFC-009   $375.00      $312.00/vial 3+      Size: 50 µg

Range: 50-200 ng/ml

Literature: [Joukov et al., EMBO J 15 :290, 1996; Kukk et al., Development 122 :3829, 1996]


For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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