rev:  November 18, 2004

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


Recombinant Human sBMPR-1A
Recombinant Human soluble BMPR-IA

Description: The extracellular domain of human BMPR-IA was fused with a carboxy -terminal 6X histidine-tag. The monomeric glycoprotein was expressed in baculovirus infected insect cells. Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor 1A (BMPR-1A), also known as activin receptor-like kinase (ALK)-3, is a one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-b family cytokines. In contrast to the TGF -b receptor system in which the type I receptor does not bind TGF-b in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IA binds BMP-2 and -4 with high-affinity in solution and is a potent BMP-2/4 antagonist in vitro. BMPR-IA is ubiquitously expressed during embryogenesis. In adult tissues, BMPR-IA mRNA is also widely distributed; with the highest expression levels found in skeletal muscle. The extracellular domain of BMPR-IA shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IA are highly conserved and share 98% sequence identity.

Source:                                   Insect cells

Molecular Weight:                 23 kDa

Subunit:                                  glycosylated monomer

Purity:                                    >90%, by SDS-PAGE and visualised by silver stain

Endotoxin level:                     < 0.1 ng per mg of sBMPR-IA

Stabilizer:                               HSA

Buffer:                                     PBS

Formulation:                            lyophilized

Biological Activity: Measured by its ability to inhibit recombinant human BMP-2 I induced alkaline phosphatase production by C2C12 myogenic cells. The ED50 for this effect is typically 1-3 µg/ml in the presence of 500 ng/ml of BMP-2.

Reconstitution: The lyophilised sBMPR-IA should be reconstituted in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.

Stability: Lyophilised samples are stable for greater than six month at -20°C to -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at -2°C to -4°C for one month or at -20°C to -70°C for three month without detectable loss of activity. Avoid repeated freeze-thaw cycles!

Usage: sBMPR-IA is offered for research use. Not for drug use. Not for human use!

Catalogue number: RDI-S01-021  Size: 100 µg  $625.00       $562.00/5+

Range: 1-4 µg/ml

Literature: [Kirsch et al., FEBS 468 :215, 2000 ; Ten Dijke et al., Oncogene 8:2879, 1993; Kawabal et al.,

Cytokine Growth Factor Rev 9:49, 1998; Ebendal et al., J Neuroscience Res 51:139, 1998]


For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


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