rev:  September 13, 2007

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Recombinant Human RECEPTORS

-the following are available from RDI for in vitro research use only. Please inquire for others along with numeorus antibodies. Shipping US$62.00/USA, $188.00 international with dry ice. Also inquire for NGFR, FGFR, PDGFBR, PDGF alpha R, and Insulin Like Growth Factor Binding Proteins. see also antibodies to various receptors.


Recombinant Human sEGFR-extracellular

Cat# RDI-EGFRX-AG     $625.00/25ug

Presentation: 25ug lyophilized from PBS (no carrier)

sequence: extracellular aa25-647 with C terminal signal peptide as shown below

Cell line: insect cells

Reconstitute: Recommend reconstitute with 0.5ml sterile DI water (50ug/ml stock), Let set at least 30 minutes, gently mix. If desired can centrifuge 1 minute 1000rpm to concentrate material in vial.

Storage: Store -20 DEG C. Upon reconstitution, recommend storing working aliquots at -20 to -70 DEG C in non-frost free freezer. Recommend keeping at >10ug/ml unless adding additional carrier protein (as 0.1-1% BSA or serum containing media).

Structure:

hsEGFR (extracellular part aa25 - 647; without signal peptide!)

LEEKKV CQGTSNKLTQ LGTFEDHFLS LQRMFNNCEV VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP LENLQIIRGN MYYENSYALA VLSNYDANKT GLKELPMRNL QEILHGAVRF SNNPALCNVE SIQWRDIVSS DFLSNMSMDF QNHLGSCQKC DPSCPNGSCW GAGEENCQKL TKIICAQQCS GRCRGKSPSD CCHNQCAAGC TGPRESDCLV CRKFRDEATC KDTCPPLMLY NPTTYQMDVN PEGKYSFGAT CVKKCPRNYV VTDHGSCVRA CGADSYEMEE DGVRKCKKCE GPCRKVCNGI GIGEFKDSLS INATNIKHFK NCTSISGDLH ILPVAFRGDS FTHTPPLDPQ ELDILKTVKE ITGFLLIQAW PENRTDLHAF ENLEIIRGRT KQHGQFSLAV VSLNITSLGL RSLKEISDGD VIISGNKNLC YANTINWKKL FGTSGQKTKI ISNRGENSCK ATGQVCHALC SPEGCWGPEP RDCVSCRNVS RGRECVDKCK LLEGEPREFV ENSECIQCHP ECLPQAMNIT CTGRGPDNCI QCAHYIDGPH CVKTCPAGVM GENNTLVWKY ADAGHVCHLC HPNCTYGCTG PGLEGCPTNG PKIPSIA

Strep-tag II (C terminal)

Ala-Ser-Trp-Ser-His-Pro-Gln-Phe-Glu-Lys (linker)

for additonal info on the Strep-Tag II system

http://www.iba-go.com/iba_us/prottools/index.php?prot_p_assay.html

For Research Use Only



recombinant human TNF Receptor p75 (Extracelluar domain)

-no longer offered, subject to patent held by Immunex #5,395,760


recombinant human TNF Receptor p55 (Extracelluar domain)

cat#RDI-TNFR55-AGE $500.00/10 micrograms    $3125.00/100 micrograms

-human TNFR (p55) extracellular domain is produced in genetically engineered insect cells (spodoptera frugiperda, Sf9) infected with recombinant virus and purified by sequential chromatography.

-Binding activity tested with competition of radioiodinated TNF.

-Purity >80% pure by SDS (Three bands are observed by SDS although a single band is observed after glycosidase treatment indicating heterogeneity in the suagr portion of this glycorpotein.

-Stable at least 2 months at -80 DEG C.

-Shipped as frozen liquid in 10mM Tris-HCL, pH 7.0. Protein concentration 1.0mg/ml.


Human NGF Receptor Extracellular Domain (recombinant)

Cat#: RDI-NGFR-AGE    $375.00/10ug    $2000.00/100ug

-Human NGF receptor extracellular domain is produced by genetically engineered Chinese hamster ovary cells (CHO cells) and purified by lectin affinity chromatography.

-Biological Activity: The human NGF receptor extracellular domainis able to blind NGF.

-Purity: The material has been judged to be >85% pure, as determined by SDS gel electrophoresis.

-Stability: Human NGF receptor extracellular domain is stable at -80'C for at least 2 months.

-Storage: Upon receipt, the material should be stored at -80'C.

-Formulation: 20mM HEPES, pH 7.3./0.1 M NaCl. Concentration: 1.0 mg protein/ml.


HUMAN PDGF ALPHA RECEPTOR EXTRACELLULAR DOMAIN (recombinant)

CAT#: RDI-PDGFA-AGE    $500.00/10ug   $3125.00/100ug
-Human PDGF alpha receptor extracellular domain is produced by genetically engineered Spodoptera frigiperda insect cells (Sf9) infected with recombinant baculovirus, and purified by lectin affinity chromatography.

-Biological Activity: The material is able to bind the ligand (PDGF) as demonstrated by immobilizing the PDGF receptor extracellular domain and establishing competition of binding with radioiodinated PDGF.

-Purity: The material has been judged to be >85% pure, as determined by SDS gel electrophoresis.

-Stability: Human PDGF receptor is stable at -80'C for at least 2 months.

-Storage: Upon receipt, the material should be stored at -80'C.

-Formulation: 10 mM Tris-HCl. pH 7.0. Concentration: 0.50 mg protein/ml.


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RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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