rev:  April 20, 2007

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Human Cytokine/Growth Factor/Chemokines Sample Spec sheets (subject to change without notice):


DATA SHEET:recombinant Human Interleukin-1 BETA

-IL-1 beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. Recombinant human IL-1 beta is a 17.3 kDa protein containing 153 amino acid residues

Catalog#: RDI-201B (formerly know as RDI-IL1B)

Package Size: 10 micrograms $235.00    $219.00/package 2 or more

                       Mini-Pack:cat#RDI-201BP $95.00/2ug

                       bulk quotes on request

Supplied: Lyophilized powder with no additives.

Source: E. Coli

Purity: >98% by SDS-Page analysis and HPLC analysis. Endotoxin level is less than 0.1 ng per ug (1EU/ug) of IL-1b.

Reconstitution: The lyophilized IL-1b is soluble in water and most aqueous buffers. The lyophilized powder can be reconstituted in water to a concentration of 0.1-1mg/ml (maximum concentration 1mg/ml). Allow to set at least 30 minutes at 4 DEG C , tighten cap and rotate to reconstitute any material on sides of vial. If desired, you can centrifuge at 1000 rpm for 1 minute to concentrate volume in bottom of vial. This solution can be diluted into other buffered solutions or stored at 4 DEG C for up to 1 week or at -20 DEG C for future use. For most in-vitro applications, IL-1b exerts its biological activity in the range of 0.1 to 10.0 ng/ml.

Note:If this material is not to be used for coating or labeling or other applications requiring carrier free material), the addition of 0.1% BSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >50ug/ml.

Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20 DEG C C. Reconstituted IL-1b should be stored in working aliquots at -20DEG C. AVOID FREQUENT FREEZE THAW CYCLES.

Reactivity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake   by murine C3H/HeJ thymocytes is less than 0.1ng/ml. This corresponds to a specific activity of 1 X 10 E07 units/mg. The ED50 as determined by the dose- dependent stimulation of thymidine uptake by murine D10S cells is <1pg/ml, corresponding to a specific activity of > 1 E09 units/mg.

AA Sequence: MAPVRSLNCT LRDSQQKSLV MSGPYELKAL HLQGQDMEQQ

                         VVFSMSFVQG EESNDKIPVA LGLKEKNLYL SCVLKDDKPT LQLESVDPKN

                        YPKKKMEKRF VFNKIEINNK LEFESAQFPN WYISTSQAEN MPVFLGGTKG

                        GQDITDFTMQ FVSS

Precautions: For In vitro research Use Only. Not for use in or on humans or animals   or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Covered under patents held by Cistron Biotechnology, Inc, U.S. Patents 4,766,069, 5,122,459 and 5,484,887.


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